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Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) shoot apices: transformation patterns

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Abstract

Apical segments of embryonic axes of sunflower (Helianthus annuus L.) embryos were submitted to co-culture experiments with a disarmed strain of Agrobacterium tumefaciens, harbouring a plasmid coding for the marker enzyme β-glucuronidase. The expression patterns of this marker were analysed at different developmental stages of the regenerated shoots. The results are consistent with the hypothesis that transformed shoots originate from transformation events that have occurred within the existing meristems. Two of the resulting chimaeric plants have been analysed in detail, and some representative gene integration patterns are presented.

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Author notes

  1. Monique Burrus

    Present address: Laboratoire de Biologie et Physiologie Végétales, UFR Sciences, Université de Reims-Champagne Ardenne, 51062, Reims Cedex, France

  2. Reiner Hunold

    Present address: Newlab, Max-Plack-Strasse 15a, 40699, Erkrath, Germany

Authors and Affiliations

  1. Institut de Biologie Moléculaire des Plantes, CNRS, 12, Rue du Général Zimmer, 67084, Strasbourg Cedex, France

    Monique Burrus, Jean Molinier, Christophe Himber, Reiner Hunold, Roberte Bronner, Philippe Rousselin & Günther Hahne

  2. Université Louis Pasteur, 12, Rue du Général Zimmer, 67084, Strasbourg Cedex, France

    Monique Burrus, Jean Molinier, Christophe Himber, Reiner Hunold, Roberte Bronner, Philippe Rousselin & Günther Hahne

Authors
  1. Monique Burrus
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  2. Jean Molinier
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  3. Christophe Himber
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  4. Reiner Hunold
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  5. Roberte Bronner
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  6. Philippe Rousselin
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  7. Günther Hahne
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Burrus, M., Molinier, J., Himber, C. et al. Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) shoot apices: transformation patterns. Mol Breeding 2, 329–338 (1996). https://doi.org/10.1007/BF00437911

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  • DOI: https://doi.org/10.1007/BF00437911

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