Abstract
A process is described for production of a cyclodepsipeptide complex (CDPC) from autoclaved white corn grits fermented with Fusarium tricinctum NRRL 3510. Yields of more than 2.5 g CDPC/ kg of medium were obtained. Previously, we described the conspicuous swelling of Pencillium digitatum conidia and hyphal tips incubating in a medium supplemented with the CDPC, a trio of metabolites produced by strains of F. tricinctum and F. roseum. Analysis of the CDPC mass spectra and the amino acid composition indicated one major and two minor cyclodepsipeptides. The two major products differed from the major cyclodepsipeptide only in the substitution of either isoleucine or valine for leucine in the threonyl-alanyl-alanyl-glutaminyl-tyrosyl-leucine peptide portion of the molecule.
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References
Burmeister, H. R., J. J. Ellis & R. F. Vesonder, 1981. Survey for Fusaria that produce an antibiotic that causes conidia of Penicillium digilatum to swell. Mycopathologia 74: 29–33.
Burmeister, H. R., R. F. Vesonder & C. W. Hesseltine, 1977. Swelling of Penicillium digitatum conidia by a Fusarium acuminaium NRRL 6227 metabolite. Mycopathologia 62: 53–56.
Carr, S. A., C. E. Costello, E. Block, R. F. Vesonder & H. R. Burmeister, 1984. Structure determination of a new cyclodepsipeptide antibiotic from Fusaria fungi. Presented at the 32nd Annual Conference on Mass Spectrometry and Allied Topics; San Antonio, Texas; May 27–June 1.
Haynes, W. C., L. J. Wickerham & C. W. Hesseltine, 1955. Maintenance of cultures of industrial importance. Appl. Microbiol. 3: 361–368.
Toussoun, T. A. & P. E. Nelson, 1976. Fusarium. 2nd Ed. Pennsylvania State University Press, University Park, Pennsylvania, 1–43.
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Burmeister, H.R., Vesonder, R.F., Peterson, R.E. et al. Production and purification of a peptide of Fusarium tricinctum that causes conidia of Penicillium to swell. Mycopathologia 91, 53–56 (1985). https://doi.org/10.1007/BF00437288
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DOI: https://doi.org/10.1007/BF00437288