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Structural characterization and temperature-dependent production of C17-fengycin B derived from Bacillus amyloliquefaciens subsp. plantarum BC32-1

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Abstract

Cyclic lipopeptides were produced by Bacillus amyloliquefaciens subsp. plantarum strain BC32-1 that was isolated from yellow loess soil in the Jeonnam province of South Korea. Several lipopeptides were isolated from the bacteria using organic solvent extraction and reverse-phase high-performance liquid chromatography (RP-HPLC). Purified iturin-, surfactin-, and fengycin-type lipopeptides were identified using liquid chromatographymass spectrometry (LC-MS) analysis. Among the lipopeptides, C17-fengycin B showed strong antifungal activity against the phytopathogenic fungus, Fusarium oxysporum f. sp. radicis-lycopersici, and then the fengycin was further characterized by UV, Fourier transform-infrared spectroscopy (FT-IR), and LC-MS/MS analyses. C17-fengycin B was highly produced at quantities of up to 15 µg/mL at 37°C, whereas little amount of the fengycin was produced at 25°C. Purified C17-fengycin B inhibited mycelial growth of F. oxysporum with a minimal inhibitory concentration of 50 µg/mL. This study suggests that C17-fengycin B is a major antifungal component produced by the BC32-1 strain that could be used as an environmentally friendly agent to control the phytopathogenic F. oxysporum.

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Authors and Affiliations

  1. Department of Chemistry, Chonnam National University, Gwangju, 500-757, Korea

    Jiyoung Nam & Chul Won Lee

  2. Bio Control Research Center, JBF, Jeonnam, 516-944, Korea

    Min Young Jung & Pyoung Il Kim

  3. Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 500-757, Korea

    Hyang Burm Lee

  4. Department of Environmental Engineering, Chosun University, Gwangju, 501-759, Korea

    Si Wouk Kim

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  1. Jiyoung Nam
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  2. Min Young Jung
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  3. Pyoung Il Kim
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  4. Hyang Burm Lee
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  5. Si Wouk Kim
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Correspondence to Si Wouk Kim or Chul Won Lee.

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Nam, J., Jung, M.Y., Kim, P.I. et al. Structural characterization and temperature-dependent production of C17-fengycin B derived from Bacillus amyloliquefaciens subsp. plantarum BC32-1. Biotechnol Bioproc E 20, 708–713 (2015). https://doi.org/10.1007/s12257-015-0350-3

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  • DOI: https://doi.org/10.1007/s12257-015-0350-3

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