Skip to main content
SpringerLink
Log in

Ultrastructure of Cryptococcus neoformans in the cerebrospinal fluid of a patient with cryptococcal meningitis

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

The ultrastructure of Cryptococcus neoformans in the cerebrospinal fluid, which was obtained from a patient suffering from systemic lupus erythematosus and cryptococcal meningitis, before treatment and on the 10th and 20th day after the start of treatment was studied. Numerous cryptococci were detected in the cerebrospinal fluid before treatment. However, in most of them their biological activity was low and their organelles were not clear. A few yeasts preserved well their organelles. Such cells showed a tendency to develop vesicular membrane structures (lomasomes) touching the internal part of the cell wall. In the cerebrospinal fluid on the 20th day all of the yeasts were dead, even though numerous yeasts were observed by an India-ink method. At this time no colonies were recovered from the fluid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bennet, J. E., W. E. Dismukes, R. J. Duma, G. Medoff, M. A. Sande, H. Gallis, J. Leonard, B. T. Fields, M. Bradshaw, H. Haywood, Z. A. McGee, T. R. Cate, C. C. Cobbs, J. F. Warner & D. W. Alling, 1974. A comparison of amphotericin B alone and combined with flucytosine in the treatment of cryptococcal meningitis. N. Engl. J. Med. 300: 126–131.

    Google Scholar 

  2. De Nollin, S. & M. Borgers, 1974. The ultrastructure of Candida albicans after in vitro treatment with miconazole. Sabouraudia 12: 341–351.

    Google Scholar 

  3. Edwards, M. R., M. Gordon, E. W. Lapa & W. C. Ghiorse, 1967. Micromorphology of Cryptococcus neoformans. J. Bacteriol. 94: 766–777.

    Google Scholar 

  4. Iwata, K., Y. Kanda, H. Yamaguchi & M. Osumi, 1973. Electron microscopic studies on the mechanism of action of clotrimazole on Candida albicans. Sabouraudia 11: 205–209.

    Google Scholar 

  5. Rippon, J. W., 1982. Cryptococcosis. In: Medical Mycology, p. 532. W. B. Saunders Co., Philadelphia.

    Google Scholar 

  6. Scherwitz, C., 1982. Ultrastructure of human cutaneous candidosis. J. Invest. Dermatol. 78: 200–205.

    Google Scholar 

  7. Snow, R. M. & W. E. Dismukes, 1975. Cryptococcal meningitis. Arch. Intern. Med. 135: 1155–1157.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Dermatology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, Japan

    M. Hiruma & S. Kagawa

Authors
  1. M. Hiruma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Kagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hiruma, M., Kagawa, S. Ultrastructure of Cryptococcus neoformans in the cerebrospinal fluid of a patient with cryptococcal meningitis. Mycopathologia 89, 5–12 (1985). https://doi.org/10.1007/BF00437126

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00437126

Keywords

Search

Navigation