Abstract
The biodegradation of wattle tannin was tested with six strains of yeasts isolated from decaying bark samples. The degradation of the condensed tannin was assayed in flasks and 2-liter jar fermentor by analysis of the residual leucoanthocyanin and flavanol groups after incubation during seven days. A strain of Candida guilliermondii (Y11) degraded rapidly flavanol groups without release of phenolic units in the fermentation broth using TLC analysis when a strain of Candida tropicalis Y65 mostly degraded the leucoanthocyanin groups with liberation of catechin. These results show for the first time the capacity of yeasts to degrade a condensed tannin suggesting an active role of these microorganisms in bark decomposition.
Resumé
La biodégradation du tanin de mimosa a été réalisée avec six souches de levures isolées d'échantillons d'écorces en décomposition. La dégradation de ce tanin condensé a été effectuée en fioles et en fermenteur de laboratoire de 2 litres en analysant les groupements leucoanthocyanine et flavanol après incubation pendant sept jours. Une des souches, Candida guilliermondii Y11 dégrade plus rapidement les groupements flavanols en ne libérant aucun composé phénolique simple visible en Chromatographie sur couche mince tandis qu'une autre souche (Candida tropicalis Y65) attaque plus rapidement les groupements leucoanthocyanine en libérant de la catéchine. Ces résultats montrent pour la première fois l'aptitude des levures à dégrader un tanin condensé et permet de leur attribuer un rôle actif dans la décomposition des écorces.
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Ötük, G., Deschamps, A.M. Dégradation d'un tanin condensé par plusieurs types de levures. Mycopathologia 83, 107–111 (1983). https://doi.org/10.1007/BF00436891
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DOI: https://doi.org/10.1007/BF00436891