Abstract
A new method is described for the quantitative microbiological analysis of the thiamine analog pyrithiamine present in biological material. The method uses a mutant of Lactobacillus fermenti (ATCC 9338) stimulated by pyrithiamine. By this specific method it is found that Serratia marcescens is able to consume and to phosphorylate the antivitamine (presumably to pyrithiamine monophosphate).
The uptake of the analog influences neither the generation time of Serratia marcescens nor the biomass of the culture, nor the total amounts of carbohydrate and protein within the cells. On the contrary, pyrithiamin stimulates the exponential death-rate k and decreases the quantity of neutral fats. The carbon source glycerol is consumed at a higher rate, which is paralleled by an increased total amount of pyruvate as well as by an increased biosynthesis of acetoin.
Zusammenfassung
Es wird ein neues Verfahren zur quantitativen Bestimmung des Antivitamins Pyrithiamin in biologischem Material als mikrobiologischer Test mit einer Pyrithiamin-stimulierten Mutante von Lactobacillus fermenti (ATCC 9338) beschrieben. Mit dieser spezifischen Methode erfolgt der Nachweis, daß Serratia marcescens das Antivitamin aufnimmt und wahrscheinlich zum Pyrithiamin-monophosphat phosphoryliert.
Durch das aufgenommene Analogon werden bei Serratia marcescens Generationszeit, Biomasse der Kultur, Gesamtkohlenhydrate und-proteine nicht verändert. Dagegen fördert Pyrithiamin die exponentielle Absterberate k und den Umsatz der C-Quelle Glycerin, korrelierend mit der Erhöhung des Gesamtpyruvats und der Biosynthese von Acetoin. Der Gehalt an Neutralfetten wird reduziert.
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Literatur
Airth, R.L., Foerster, G.E.: Simultaneous determination of thiamine and pyrithiamine. Analyt. Biochem. 3, 383–395 (1962)
Amaraki, S.: On the biological effect of ethylthiamine. Vitamin 17, 117–123 (1959)
Amaraki, S., Nakamura, S.: On the biological effect of ethylthiamine. II. Cocarboxylase activity of ethylthiamine pyrophosphate. Vitamin 18, 393–399 (1959)
Bartnicki-Garcia, S., Nickerson, W.J.: Thiamine and nicotinic acid: anaerobic growth factors for Mucor rouxii. J. Bact. 82, 142–148 (1961)
Bolliger, H.R., König, A.: Vitamine, einschließlich Carotinoide, Chlorophylle und biologisch aktive Chinone. In: Dünnschichtchromatographie, 2. Aufl., E. Stahl, Hrsg. Berlin-Heidelberg-New York: Springer 1967
Breed, R.S., Murray, E.G.D., Smith, N.R.: Bergey's manual of determinative bacteriology, 7th ed. Baltimore: Williams and Wilkins 1957
Das, S.K., Chatterjee, G.C.: Pyrithiamine adaptation of Staphylococcus aureus. I. Adaptation and carbohydrate utilization. J. Bact. 83, 1251–1259 (1962)
Douthit, H.A., Airth, R.L.: Thiaminase I of Bacillus thiaminolyticus. Arch. Biochem. Biophys. 133, 331–337 (1966)
Eschenbruch, R., Dittrich, H.H.: Die Acetoinbildung von Lactobacillus plantarum in Abhängigkeit von Thiamin, Liponsäure, l-Valin und l-Isoleucin. Arch. Mikrobiol. 70, 303–312 (1970)
Gubler, C.J.: Enzyme studies in thiamine deficiency. Int. Z. Vitaminforsch. 38, 287–303 (1968)
Hagedorn, H.: Experimentelle Untersuchungen über den Einfluß des Thiamins auf die natürliche Algenpopulation des Pelagials. Arch. Hydrobiol. 68, 382–399 (1971a)
Hagedorn, H.: Die ökologische Bedeutung des Thiamins. Ber. dtsch. bot. Ges. 84, 479–482 (1971b)
Johnson, D.B., Goodwin, T.W.: α-Hydroxyethylthiamine in plant tissues. Biochem. J. 88, 62–63 (1963)
Johnson, L.R., Gubler, C.J.: Studies on the physiological functions of thiamine. III. The phosphorylation of thiamine in brain. Biochim. biophys. Acta (Amst.) 156, 85–86 (1968)
Kawasaki, T., Miyata, I., Esaki, K., Nose, Y.: Thiamine uptake in Escherichia coli. I. General properties of thiamine uptake system in Escherichia coli. Arch. Biochem. Biophys. 131, 223–230 (1969)
Kaziro, Y.: Studies on thiaminokinase from baker's yeast. J. Biochem. 46, 1523–1529 (1959)
Koedam, J.C., Steyn-Parvé, E.P.: The action of thiamine antagonists upon some enzymes involved in thiamine metabolism. Ned. Akad. v. Wetensch., Proc. Ser. C 63, 318–329 (1960)
Morita, M., Kanaya, T., Minesita, T.: A method for the simultaneous determination of pyrithiamine and thiamine in biological materials. J. Vitaminol. 14, 67–76 (1968a)
Morita, M., Kanaya, T., Minesita, T.: Uptake of pyrithiamine by tissues of rats in relation to tissue thiamine levels. J. Vitaminol. 14, 77–82 (1968b)
Rindi, G., Perri, V.: Uptake of pyrithiamine by tissue of rats. Biochem. J. 80, 214–216 (1961)
Sarett, H.P., Cheldelin, V.H.: Inhibition of utilization of thiamine and dephosphothiamine for growth of microorganisms. J. biol. Chem. 156, 91–100 (1944)
Schmidt, O.R.: Einfluß von Vitamin B1 auf das auxoautotrophe Bacterium Serratia marcescens. Diss., Fachbereich Biol., Westf. Wilhelms-Univ. (1973)
Schmidt, O.R., Hagedorn, H.: Der Einfluß des Thiamins auf den Stoffwechsel der thiaminautotrophen Serratia marcescens. Zbl. Bakt., II. Abt. (in press, 1974)
Schwarz, D.: Entstehung des Thiamins und seine Bedeutung für Bakterien und Algen in aquatischen Biotopen. Veröff. des Instituts f. Wasserforschung, Dortmund (1972)
Shimazono, N., Mano, Y., Kaziro, Y., Nakamura, S., Aramaki, S., Kobayashi, M.: Thiamine antagonists. Vitamin 19, 553–559 (1960)
Sinha, A.K., Chatterjee, G.C.: Metabolism of pyrithiamine by the pyrithiamine-requiring mutant of Staphylococcus aureus. Biochem. J. 107, 165–169 (1968)
Sompolinsky, D., Neujahr, H.Y.: Studies of thiamine uptake in growing cultures and in cell fragments of Lactobacillus fermenti. Acta chem. scand. 25, 3054–3066 (1971)
Telegdy-Kováts, M.: Growth promoting effect of oxythiamine on Lactobacillus fermenti. Int. Z. Vitaminforsch. 37, 329–330 (1967)
Wang, L., Wilkins, J.H., Airth, R.L.: Repression of thiaminase I by thiamine and related compounds in Bacillus thiaminolyticus. Canad. J. Microbiol. 14, 1143–1147 (1968)
Witt, I., Neufang, B.: Studies on the influence of thiamine on the synthesis of thiamine pyrophosphate-dependent enzymes in Saccharomyces cerevisiae. Biochim. biophys. Acta (Amst.) 215, 323–332 (1970)
Wittorf, J.H., Gubler, C.J.: Coenzyme binding in yeast pyruvate decarboxylase. Kinetic studies with thiamine diphosphate analogues. Europ. J. Biochem. 22, 544–550 (1971)
Woolley, D.N., White, A.G.C.: Selective reversible inhibition of microbial growth with pyrithiamine. J. exp. Med. 98, 489–495 (1943)
Wolley, E.Y. DW.: A Study of antimetabolites. New York: Wiley 1952
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Hagedorn, H., Schmidt, O.R. Die Wirkung des Antivitamins Pyrithiamin auf den Stoffwechsel der thiaminautotrophen Serratia marcescens . Arch. Microbiol. 103, 83–88 (1975). https://doi.org/10.1007/BF00436334
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DOI: https://doi.org/10.1007/BF00436334