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Dependence of growth characteristics of original strains of Lactococcus lactis subsp. lactis on the composition of agar media used for biomass growth

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Abstract

Data on the dependence of growth characteristics of Lactococcus lactis subsp. lactis 729 and TB2 extracted from milk and homemade curd on the content of casein peptone (CP) as the main source of nitrogen in the agar medium is presented. The number of colony-forming units (CFUs), cell division rate (CDR), and mean hourly doubling time (HDT) were compared between two time segments of cultivation: from 1 to 2.5 h, and from 2.5 to 3.5 h. In liquid media, logarithmic growth was observed for the 729 strain in all groups for 3.5 h at minimal pH of 6.5. The maximum biomass production rate (2.5 × 108 cells/mL) was observed in the media containing the meat extract; the minimal HDT was in the biomass transferred from the minimal MA4 medium to the enriched M7 medium. In contrast with the TB2 strain, the 729 strain does not form colonies on agar media at CP concentrations below 0.4%. The colonies of the TB2 strain increase in size from 2.85 mm2 to 5 mm2 when decreasing CP concentration from 1 to 0.4%. The yeast extract has a stimulating effect in liquid media at said concentration ratios, leading to an increase in the efficiency of cell division at lower rates of lactic acid production, to pH of 5.5 when oligopeptide transport is blocked. The meat extract provides adaptation of bacteria to the conditions of excessive acidity and has positive effect on cell division in the 729 strain at later stages of bacterial colony development. The smaller size of colonies of the strain might be evidence of active acid formation.

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Authors and Affiliations

  1. Ivanovsky Institute of Virology, ul. Gamalei 16, Moscow, 123098, Russia

    M. A. Trenina

  2. Scientific and Production Center “Armbiotechnology,”, National Academy of Sciences of Republic of Armenia, Yerevan, 0056, Republic of Armenia

    A. S. Yepremyan

  3. Department of Biology, Moscow State University, Moscow, 119991, Russia

    L. G. Stoyanova

Authors
  1. M. A. Trenina
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  2. A. S. Yepremyan
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  3. L. G. Stoyanova
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Correspondence to L. G. Stoyanova.

Additional information

Original Russian Text © M.A. Trenina, A.S. Yepremyan, L.G. Stoyanova, 2015, published in Vestnik Moskovskogo Universiteta. Biologiya, 2015, No. 1, pp. 31–36.

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Trenina, M.A., Yepremyan, A.S. & Stoyanova, L.G. Dependence of growth characteristics of original strains of Lactococcus lactis subsp. lactis on the composition of agar media used for biomass growth. Moscow Univ. Biol.Sci. Bull. 70, 29–34 (2015). https://doi.org/10.3103/S0096392515010095

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  • DOI: https://doi.org/10.3103/S0096392515010095

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