Skip to main content
SpringerLink
Log in

Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219

  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that strains completely lacking 2 µm DNA sequences can be obtained routinely.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beggs JD (1978) Nature 275:104–109

    Google Scholar 

  • Blanc H, Gerbaud C, Slonimski PP, Guerineau M (1979) Mol Gen Genet 176:335–342

    Google Scholar 

  • Broach JR, Strathern JN, Hicks JB (1979) Gene 8:121–133

    Google Scholar 

  • Clark-Walker GD (1972) Proc Natl Acad Sci USA 69:388–392

    Google Scholar 

  • Clark-Walker GD, Miklos GLG (1974) Eur J Biochem 41:359–365

    Google Scholar 

  • Cox BS (1965) Heredity 20:505–521

    Google Scholar 

  • Dobson M, Futcher AB, Cox BS (1980) Curr Gen 2:193–200

    Google Scholar 

  • Garvik B, Haber JE (1978) J Bacteriol 134:261–269

    Google Scholar 

  • Gerbaud C, Guerineau M (1980) Curr Gen 1:219–228

    Google Scholar 

  • Griffiths DE, Lancashire WE, Zanders ED (1975) FEBS Lett 53:126–130

    Google Scholar 

  • Gubbins EJ, Newlon CS, Kann MD, Donelson JE (1977) Gene 1:185–207

    Google Scholar 

  • Guerineau M, Grandchamp C, Slonimski PP (1976) Proc Natl Acad Sci USA 73:3030–3034

    Google Scholar 

  • Guerineau M, Slonimski PP, Avner PR (1974) Biochem Biophys Res Commun 61:462–469

    Google Scholar 

  • Lacroute F (1971) J Bacteriol 106:519–522

    Google Scholar 

  • Livingston DM (1977) Genetics 86:73–84

    Google Scholar 

  • Saunders GW, Rank GH, Kustermann-Kuhn B, Hollenberg CP (1979) Mol Gen Genet 175:45–52

    Google Scholar 

  • Sinclair JH, Stevens RJ, Sanghavi P, Rabinowitz M (1967) Science 156:1234–1237

    Google Scholar 

  • Storms RK, McNeil JB, Khandekar PS, Parker J, An GJ, Friesen JD (1979) J Bacteriol 140:73–82

    Google Scholar 

  • Struhl K, Stinchcomb DT, Scherer S, Davis RW (1979) Proc Natl Acad Sci USA 76:1035–1039

    Google Scholar 

  • Tabak HF (1977) FEBS Letters 84:67–70

    Google Scholar 

  • Toh-e A, Guerry-Kopecko P, Wickner RB (1980) J Bacteriol 141:413–416

    Google Scholar 

  • Wickner RB (1980) Cell 21:217–226

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Botany School, Sóuth Parks Road, 0X1 3RA, Oxford, England

    Melanie J. Dobson, A. Bruce Futcher & Brian S. Cox

Authors
  1. Melanie J. Dobson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Bruce Futcher
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Brian S. Cox
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dobson, M.J., Futcher, A.B. & Cox, B.S. Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219. Curr Genet 2, 201–205 (1980). https://doi.org/10.1007/BF00435686

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00435686

Key words

Search

Navigation