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Isolation and characterization of a methylammonium resistant mutant of Neurospora crassa

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Summary

A mutant of Neurospora crassa has been isolated which is resistant to methylammonium, a structural analog of ammonium. In contrast to wild type, this mutant, mea-1, has derepressed nitrate reductase and nitrite reductase activities in the presence of ammonium. However, glutamine still represses these nitrate assimilation enzymes in mea-1. The nit-2 mutant was epistatic to mea-1 since the mea-1; nit-2 double mutant has the nit-2 mutant phenotype. In addition, mea-1; nit-2 double mutants cannot utilize ammonium as a nitrogen source. We suggest therefore that nit-2 and mea-1 loci play a role in ammonia/methylamine uptake.

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Author notes

  1. Nigel S. Dunn-Coleman

    Present address: Central Research and Development Department, Visiting Scientist, Du Pont Experimental Station, 19898, Wilmington, Delaware, USA

Authors and Affiliations

  1. Department of Biology, Gilmer Hall, University of Virginia, 22901, Charlottesville, Virginia, USA

    Nigel S. Dunn-Coleman, Marie D. Nassiff & Reginald H. Garrett

Authors
  1. Nigel S. Dunn-Coleman
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  2. Marie D. Nassiff
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  3. Reginald H. Garrett
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Dunn-Coleman, N.S., Nassiff, M.D. & Garrett, R.H. Isolation and characterization of a methylammonium resistant mutant of Neurospora crassa . Curr Genet 8, 423–427 (1984). https://doi.org/10.1007/BF00433908

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  • DOI: https://doi.org/10.1007/BF00433908

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