Summary
The order of eight of the 29 endonuclease EcoRI-generated fragments of chloroplast DNA was determined. Three sets of rRNA genes aligned sequentially in the same orientation form part of this region. The repeated sets differ in the length and sequence of the spacers among themselves and with the rRNA genes of E. gracilis strain Z.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adams, J., Kinney, T., Thompson, S., Rubin, L., Helling, R.B.: Frequency-dependent selection for plasmid-containing cells of Escherichia coli. Genetics 91, (1979)
Bedbrook, J.R., Kolodner, R., Bogarad, L.: Zea mays chloroplast ribosomal RNA genes are part of a 22000 base pair inverted repeat. Cell 11, 739–749 (1977)
Carroll, D., Brown, D.D.: Adjacent repeating units of Xenopus laevis 5S DNA can be heterogeneous in length. Cell 7, 477–486 (1976)
Denhardt, D.T.: A membrane-filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun. 23, 641–646 (1966)
Gray, P.W., Hallick, R.B.: Physical mapping of the Euglena gracilis chloroplast DNA and ribosomal RNA gene region. Biochemistry 17, 284–289 (1978)
Gray, P.W., Hallick, R.B.: Restriction endonuclease map of Euglena gracilis chloroplast DNA. Biochemistry 16, 1665–1671 (1977)
Gruol, D.J., Haselkorn, R.: Counting the genes for stable RNA in the nucleus and chloroplasts of Euglena. Biochim. Biophys. Acta 447, 82–95 (1976)
Helling, R.B., Goodman, H.M., Boyer, H.W.: Analysis of endonuclease R·EcoRI fragments of DNA from lambdoid bacteriophage and other viruses by agarose-gel electrophoresis. J. Virol. 14, 1235–1244 (1974)
Helling, R.B., Lomax, M.I.: The molecular cloning of genes—general procedures. In: Genetic engineering (A. Chakrabarty, ed.). W. Palm Beach: CRC Press 1978
Knopf, V.C., Stutz, E.: Molecular cloning of the gene region coding for the chloroplast rRNA of Euglena gracilis. Mol. Gen. Genet. 163, 1–6 (1978)
Laskey, R.A., Mills, A.D.: Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. J. Biochem. 56, 335–341 (1975)
Lomax, M.I., Helling, R.B., Hecker, L.I., Schwartzbach, S.D., Barnett, W.E.: Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis. Science 196, 202–205 (1977)
Manning, J.E., Richards, O.C.: Isolation and molecular weight of circular chloroplast DNA from Euglena gracilis. Biochim. Biophys. Acta 259, 285–296 (1972)
McCrea, J.M., Hershberger, C.L.: Chloroplast DNA codes for transfer RNA. Nucleic Acids Res. 3, 2005–2018 (1976)
McDonell, M.W., Simon, M.N., Studier, F.W.: Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J. Mol. Biol. 110, 119–146 (1977)
Mielenz, J.R., Milner, J.J., Hershberger, C.L.: Analysis of Euglena gracilis chloroplast deoxyribonucleic acid with a restriction endonuclease, EcoRI. J. Bacteriol. 130, 860–868 (1977)
Peacock, A.C., Dingman, C.W.: Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7, 668–674 (1968)
Rawson, J.R.A., Boerma, C.L.: A measurement of the fraction of chloroplast DNA transcribed during chloroplast development in Euglena gracilis. Biochemistry 15, 588–592 (1976)
Rawson, J.R.Y., Kushner, S.R., Vapnek, D., Alton, N.K., Boerma, C.L.: Chloroplast ribosomal RNA genes in Euglena gracillis exist as three clustered tandem repeats. Gene 3, 191–209 (1978)
Rigby, P.W.J., Dieckmann, M., Rhodes, C., Berg, P.: Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113, 237–251 (1977)
Rochaix, J.D., Malnoe, P.: Anatomy of the chloroplast ribosomal DNA of Chlamydomonas reinhardii. Cell 15, 661–670 (1978)
Schwartzbach, S.D., Hecker, L.I., Barnett, W.E.: Transcriptional origin of Euglena chloroplast tRNAs. Proc. Natl. Acad. Sci. U.S.A. 73, 1984–1988 (1976)
Scott, N.S.: Precursors of chloroplast ribosomal RNA in Euglena gracilis. Phytochemistry 15, 1207–1213 (1976)
Slavick, N.S., Hershberger, C.L.: The kinetic complexity of Euglena gracilis chloroplast DNA. FEBS Lett. 52, 171–174 (1975)
Southern, E.M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517 (1975)
Wellauer, P.K., Dawid, I.B., Brown, D.D., Reeder, R.H.: The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevis. J. Mol. Biol. 105, 461–486 (1976)
Whitfield, P.R., Herrmann, R.G., Bottomley, W.: Mapping of the ribosomal RNA genes on spinach chloroplast DNA. Nucleic Acids Res. 5, 1741–1751 (1978)
Author information
Authors and Affiliations
Additional information
Communicated by H. Boyer
Rights and permissions
About this article
Cite this article
Helling, R.B., El-Gewely, M.R., Lomax, M.I. et al. Organization of the chloroplast ribosomal RNA genes of Euglena gracilis bacillaris . Molec. Gen. Genet. 174, 1–10 (1979). https://doi.org/10.1007/BF00433298
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00433298