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A regulatory gene and a structural gene for alaninase in Escherichia coli

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Summary

Two genes involved in the enzymatic conversion of D-alanine to pyruvate are described, alnA and alnR. The alnA gene, located between ara and leu, is probably the structural gene for alaninase. The alnR gene, which can be cotransduced with thr but not with leu, could be demonstrated to be a regulatory gene with the aid of a mutation resulting in permanent repression and a thermosensitive revertant of this mutation restoring inducibility at 28°C, but not at 42°C.

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Authors and Affiliations

  1. Institute of Genetics, University of Amsterdam, Amsterdam, the Netherlands

    R. H. J. Beelen, A. M. Feldmann & H. J. W. Wijsman

Authors
  1. R. H. J. Beelen
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  2. A. M. Feldmann
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  3. H. J. W. Wijsman
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Communicated by W. Maas

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Beelen, R.H.J., Feldmann, A.M. & Wijsman, H.J.W. A regulatory gene and a structural gene for alaninase in Escherichia coli . Molec. Gen. Genet. 121, 369–374 (1973). https://doi.org/10.1007/BF00433235

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  • DOI: https://doi.org/10.1007/BF00433235

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