Summary
The isolation of λ transducing phages carrying the tolPAB cluster is described. These genes map between gltA and gal in Escherichia coli, and thus are relatively close to attλ. To isolate these transducing phages, it was necessary to use a strain deleted of most of the intervening genes (nadA to chlD) between tolPAB and attλ. Using a lysogen of such a deletion strain, several defective λdtol phages were isolated that carry different amounts of the tolPAB cluster.
All of these λdtolPAB phages were defective in both lysogenization and vegetative growth, and in this respect were similar to λdgal transducing phages.
The usefulness of such specialized transducing phages in studying the cell surface is discussed.
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Communicated by M. Nomura
Research Fellow of the National Cancer Institute of Canada.
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Bernstein, A., Rolfe, B. & Onodera, K. The E. coli cell surface: Isolation of λ transducing phages carrying the tolPAB cluster. Molec. Gen. Genet. 121, 325–335 (1973). https://doi.org/10.1007/BF00433231
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DOI: https://doi.org/10.1007/BF00433231