Abstract
The uptake of 14C-dopamine (DA) by human platelets at 10-7–2.5×10-4 M of labelled amine concentration was studied in human platelet-rich plasma. The total uptake could be resolved into two components, one of which was saturable and completely inhibited by 10-5 imipramine and another which was unsaturable but temperature-dependent. The saturable uptake of DA had an apparent Km of 75×10-6 M and V max of 1.34 pmol/106 platelets/min. The uptake of unsaturable DA was 1.33 pmol/106 platelets/min at 10-4 M DA.
Dopamine exerted a mixed non-competitive inhibition of the saturable 5-HT transport and vice versa. Thus the increase in Km was paralleled by a decrease in V max.
The low-affinity transport of DA by the human platelets does not share any of the dopamine uptake characteristics found in neuronal tissue. The platelet therefore seems to be a poor model for the presynaptic function of the dopamine neurons.
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Malingren, R. Platelets and biogenic amines. Psychopharmacology 84, 480–485 (1984). https://doi.org/10.1007/BF00431453
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DOI: https://doi.org/10.1007/BF00431453