Abstract
Pectic polysaccharides in the cell wall of suspension-cultured carrot cells (Daucus carota L.) were fractionated into high- and low-molecular-weight components by molecular-sieve chromatography with a Sepharose 4B column. During the phase of cell-wall expansion, the relative content of low-molecular-weight polymers rapidly increased. Electrophoretic analyses of these fractions showed that the high-molecular-weight components were largely composed of neutral and weakly acidic polymers while the low-molecular-weight fraction contained, in addition to neutral polymers, strongly acidic polyuronides in which the content of neutral sugars was very small. The accumulation of a large amount of the strongly acidic polyuronides occurred in a late stage of cell-wall growth, concomitant with a marked decrease in the high-molecular-weight components.
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Abbreviations
- MW:
-
molecular weight
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Asamizu, T., Nakayama, N. & Nishi, A. Pectic polysaccharides in carrot cells growing in suspension culture. Planta 160, 469–473 (1984). https://doi.org/10.1007/BF00429765
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DOI: https://doi.org/10.1007/BF00429765