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Studies on the release of barley aleurone cell proteins: Kinetics of labelling

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Summary

Protein release from gibberellic acid-treated aleurone cells of barley (Hordeum vulgare L.) was followed in pulse-chase experiments with radioactively labelled amino acids. After a 10-min pulse of [3H]leucine or [3H]tryptophan, label was incorporated into trichloroacetic-acid (TCA)-insoluble material; some of this was released into the incubation medium during a chase with carrier amino acid. This relase of TCA-insoluble material into the incubation medium had no appreciable lag period. Precipitation with rabbit-anti-α-amylase antibody of the radioactivity released from aleurone layers into the medium during chasing indicates that as much as 70% of the radioactivity present is α-amylase. Aleurone cell homogenates were fractionated by differential centrifugation after pulsing with labelled amino acids. Radioactivity in TCA-insoluble materials was distributed equally among all sediment fractions indicating that no specific accumulation of label occurred. Tissue was also fractionated after labelling with a pulse of [3H]tyrosine and [14C]-tryptophan, and the distribution of radioactivity in various fractions also showed that no preferential sedimentation of label occurred. Altogether, no experimental evidence could be found to support the hypothesis that proteins are released from aleurone cells via discrete secretory organelles.

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References

  • Chrispeels, M. J.: Synthesis and secretion of hydroxyproline containing macromolecules in carrots. I. Kinetic Analysis. Plant Physiol. 44, 1187–1193 (1969)

    Google Scholar 

  • Chrispeels, M. J., Varner, J. E.: Gibberellic acid-enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol. 42, 398–406 (1967)

    Google Scholar 

  • Cunningham, W. P., Morrè, D. J., Mollenhauer, H. H.: Structure of isolated plant Golgi apparatus revealed by negative staining. J. Cell Biol. 28, 169–179 (1966)

    Google Scholar 

  • Evins, W. H.: Enhancement of polyribosome formation and induction of tryptophan-rich proteins by gibberellic acid. Biochemistry 10, 4295–4303 (1971)

    Google Scholar 

  • Evins, W. H., Varner, J. E.: Hormone controlled synthesis of endoplasmic reticulum in barley aleurone cells. Proc. nat. Acad. Sci. (Wash.) 68, 1631–1633 (1971)

    Google Scholar 

  • Evins, W. H., Varner, J. E.: Hormone control of polyribosome formation in barley aleurone layers. Plant Physiol. 49, 348–352 (1972)

    Google Scholar 

  • Farr, R. S.: A quantitative immunochemical measure of the primary interaction I*BSA and antibody. J. infect Dis. 103, 239–262 (1958)

    Google Scholar 

  • Fischer, E. H., Stein, E. A.: α-Amylase In: Enzymes, vol. 4, p. 313–343, P. D. Boyer, H. Lardy, K. Myrbäck, eds. New York:Acad. Press 1960

    Google Scholar 

  • Gibson, R. A., Paleg, L. G.: Lysosomal nature of hormonally induced enzymes in wheat aleurone cells. Biochem. J. 128, 367–375 (1972)

    Google Scholar 

  • Jamieson, J. D., Palade, G. E.: Role of the Golgi complex in the intracellular transport of secretory proteins. Proc. nat. Acad. Sci. (Wash.) 55, 424–431 (1966)

    Google Scholar 

  • Jamieson, J. D., Palade, G. E.: Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex. J. Cell Biol. 34, 557–596 (1967)

    Google Scholar 

  • Johnson, K. D., Kende, H.: Hormonal control of lecithin synthesis in barley aleurone cells: regulation of the CDP-choline pathway by gibberellin. Proc. nat. Acad. Sci. (Wash.) 68, 2674–2677 (1971)

    Google Scholar 

  • Jones, R. L.: Gibberellic acid and the fine structure of barley aleurone cells. I. Changes during the lag phase of α-amylase synthesis. Planta (Berl.) 87, 119–133 (1969a)

    Google Scholar 

  • Jones, R. L.: Gibberellic acid and the fine structure of barley aleurone cells. II. Changes during the synthesis and release of α-amylase. Planta (Berl.) 88, 73–86 (1969b)

    Google Scholar 

  • Jones, R. L.: Fractionation of the enzymes of the barley aleurone layer: Evidence for a soluble mode of enzyme release. Planta (Berl.) 103, 95–109 (1972)

    Google Scholar 

  • Jones, R. L., Price, J. M.: Gibberellic acid and the fine structure of barley aleurone cells. III. Vacuolation of the aleurone cell during the phase of ribonuclease release. Planta (Berl.) 94, 191–202 (1970)

    Google Scholar 

  • Jones, R. L., Varner, J. E.: The bioassay of gibberellins. Planta (Berl.) 72, 53–59 (1967)

    Google Scholar 

  • Levy, H. B., Sober, H. A.: A simple chromatographic method for preparation of gamma globulin. Soc. exp. biol. Med. 103, 25–252 (1960)

    Google Scholar 

  • Liebow, C., Rothman, S. S.: Membrane transport of proteins. Nature (Lond.) 240, 176–177 (1972)

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement within the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)

    Google Scholar 

  • Melcher, U., Varner, J. E.: Protein release by barley aleurone layers. J. Inst. Brew. 77, 456–461 (1971)

    Google Scholar 

  • Vigil, E., Ruddat, M.: Effect of gibberellic acid and actinomycin D on the formation and distribution of rough endoplasmic reticulum in barley aleurone cells. Plant Physiol. 51, 549–558 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany, University of California, 94720, Berkeley, California, USA

    Ru-fang Chen & Russell L. Jones

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  1. Ru-fang Chen
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  2. Russell L. Jones
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Chen, Rf., Jones, R.L. Studies on the release of barley aleurone cell proteins: Kinetics of labelling. Planta 119, 193–206 (1974). https://doi.org/10.1007/BF00429044

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