Advertisement

Archives of Microbiology

, Volume 108, Issue 2, pp 227–230 | Cite as

Restriction in Myxococcus virescens

  • D. W. Morris
  • J. H. Parish
Article

Abstract

  1. 1.

    The plating efficiency of bacteriophage MX-1 on Myxococcus xanthus strains A and B and M. virescens V2 were compared. Comparison of strains V2 and A suggest that V2 is restrictive and A is not (restriction coefficient was approximately 8). A derivative of M. virescens V2 (strain V2-9) was obtained by repeated exposure of strain V2 to ultraviolet radiation. Strain V2-9 was also unrestrictive. Strain B is apparently unrestrictive too but analysis of phenotypic changes in phage derived from hosts V2, B and A suggested that some of the host-cell processes differ from orthodox restriction and modification.

     
  2. 2.

    Cell-free extracts from M. virescens V2 were fractionated by ion-exchange chromatography and two restriction endonucleases, R. MviV2I and R. MviV2II were identified. Nuclease I was found to hydrolyse coliphage λ DNA at apparently one site only and MX-1 DNA at approximately 10 sites; nuclease II was found to hydrolyse MX-1 DNA at a very large number of sites and its restriction sequence was of comparable frequency with that of R. EcoRII. “Modified MX-1 DNA”, obtained from phage whose last host was M. virescens V2 was hydrolysed by nuclease II but not by nuclease I. The significance of these findings for restriction in myxococci is discussed.

     

Key words

Bacteriophage MX-1 Myxococcus virescens Myxococcus xanthus Restriction Modification 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arber, W.: DNA modification and restriction. Prog. Nuc. Acid Res. molec. Biol. 14, 1–37 (1974)Google Scholar
  2. Arber, W., Wauters-Willems, D.: Host specificity of DNA produced by Escherichia coli. XII. The two restriction and modification system of strain 15 T. Molec. gen. Genet. 108, 203–217 (1970)Google Scholar
  3. Brown, N. L., Burchard, R. P., Morris, D. W., Parish, J. H., Stow, N. D., Tsopanakis, Ch.: Phage and defective phage of strains of Myxococcus. Arch. Microbiol. 108, in press, 1976a)Google Scholar
  4. Brown, N. L., Morris, D. W., Parish, J. H.: DNA of Myxococcus bacteriophage MX-1: Macromolecular properties and restriction fragments. Arch. Microbiol. 108, 221–226 (1976b)Google Scholar
  5. Burchard, R. P., Dworkin, M.: A bacteriophage for Myxococcus xanthus: Isolation, characterization and relation of infectivity to host morphogenesis. J. Bact. 91, 1305–1313 (1966)Google Scholar
  6. Davidson, N., Szybalski, W.: Physical and chemical characteristics of lambda DNA. In: The bacteriophage lambda (A. D. Hershey, ed.), pp. 45–82. Cold Spring Harbor Monographs. New York: Cold Spring Harbor 1971Google Scholar
  7. Jeppeson, P. G. N.: A method for separating DNA fragments by electrophoresis in polyacrylamide concentration gradient slab gels. Analyt. Biochem. 58, 195–207 (1974)Google Scholar
  8. Murray, N. E., Murray, R.: Manipulation of restriction targets in phage λ to form receptor chromosomes for DNA fragments. Nature (Lond.) 251, 476–481 (1974)Google Scholar
  9. Parish, J. H.: Transfer of drug resistance to Myxococcus from bacteria carrying drug-resistance factors. J. gen. Microbiol. 87, 198–210 (1975)Google Scholar
  10. Parish, J. H., Wedgwood, K. R., Herries, D. G.: Morphogenesis in Myxococcus xanthus and Myxococcus virescens (Myxobacterales). Arch. Microbiol. 107, 343–351 (1976)Google Scholar
  11. Roberts, R. J., Breitmeyer, J. B., Tabachnik, N. F., Myers, P. A.: A second specific endonuclease from Haemophilus aegyptius. J. molec. Biol. 91, 121–123 (1975)Google Scholar
  12. Smith, H. O., Nathans, D.: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. J. molec. Biol. 81, 419–423 (1975)Google Scholar
  13. Studier, F. W.: Gene 0.3 of bacteriophage T7 acts to overcome-the DNA restriction system of the host. J. molec. Biol. 94, 283–295 (1975)Google Scholar
  14. Tsopanakis, C., Parish, J. H.: Bacteriophage MX-1: Properties of the phage and its structural proteins. J. gen. Virol. 30 (in press, 1976)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • D. W. Morris
    • 1
  • J. H. Parish
    • 1
  1. 1.Department of BiochemistryUniversity of LeedsLeedsUK

Personalised recommendations