Summary
The temperate actinophage SH10 mediates generalized transduction in Streptomyces hygroscopicus at low frequency. The efficiency of transduction depends on the average phage input, age of outgrowing spores of the recipient and on the selective marker. The highest EOT was found for the auxotrophic mutants 21 (phe-) and 5(try-)(4.1x10-6 and 2.7x10-6, respectively). Transduction of the thermosensitive mutant NG14-216 ts 35 was two orders of magnitude lower (2.5x10-8). The transductant colonies segregated into stable and unstable clones. Stable transductants were never found to be lysogenic for phage SH10.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adams MH (1959) In: Bacteriophages. Interscience Publishers, Inc. New York
Alačevic M (1963) Interspecific recombination in Streptomyces. Nature 197:1323
Alikhanian SI, Ilyina TS, Lomovskaya ND (1960) Transduction in actinomycetes. Nature 188:245–246
Bott K, Strauss B (1965) The carrier state of Bacillus subtilis infected with the transducing bacteriophage SP10. Virology 25:212–225
Campos JM, Geisselsoder J, Zusman DR (1978) Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus. J Mol Biol 119:167–178
Chater KF, Carter AT (1979) A new wide host-range, temperate bacteriophage (R4) of Streptomyces and its interaction with some restriction-modification systems. J Gen Microbiol 115:431–442
Chelala CA, Margolin P (1974) Effects of deletions on cotransduction linkage in Salmonella typhimurium: Evidence that bacterial chromosome deletions affect the formation of transducing DNA fragments. Mol Gen Genet 131:97–112
Danilenko VN, Lomovskaya ND, Alikhanian SI (1975) Properties of actinomycete hybride between Streptomyces coelicolor A3(2) and Streptomyces lividans 66. Genetika 11:67–78
Dowding JE (1972) Studies on bacteriophages of Streptomyces coelicolor, PhD Thesis, University of East Anglia, Norwich
Drexler H (1970) Transduction by bacteriophage Tl. Proc Natl Acad Sci USA 66:1083–1088
Eisenstark A (1967) Bacteriophage techniques. In: Methods in Virology, vol 1. Academic Press, New York
Godfrey O, Ford L, Huber MLB (1978) Interspecies matings of Streptomyces fradiae with Streptomyces bikiniensis mediated by conventional and protoplast fusion techniques. Can J Microbiol 24:994–997
Hopwood DA (1967) Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev 31:373–403
Hopwood DA, Chater KF, Dowding JE, Vivian A (1973) Advances in Streptomyces coelicolor genetics. Bacteriol. Rev. 37:371–405
Hopwood DA, Merrick MJ (1977) Genetics of antibiotic production. Bacteriol Rev 41:595–635
Klaus S, Süss F, Juch C, Hauck A (1978) Characterization of the virulent actinophage S2. Z Allg Mikrobiol18:575–586
Klaus S, Triebel H, Hartmann M, Walter A, Walter F, Zöpel P, Bär H (1979) Molecular characterization of the genomes of actinophage SH3, SH10, SH11, and SH12 infecting Streptomyces hygroscopicus. Mol Gen Genet 172:319–327
Klaus S, Krügel H, Süss F, Neigenfind M, Zimmermann I, Taubeneck U (1981) Properties of the temperate actinophage SH10. J Gen Microbiol (in press)
Lomovskaya ND, Mrktumian NM, Gostimskaya NL, Danilenko VN (1972) Characterization of temperate acinophage øC31 isolated from Streptomyces coelicolor A3(2). J Virol 9:258–262
Lomovskaya ND, Voeykova TA, Mrktumian NM (1977) Construction and properties of hybrids obtained in interspecific crosses between Streptomyces coelicolor A3(2) and Streptomyces griseus KR15. J Gen Microbiol 98:187–198
Malke H (1969) Transduction of Streptococcus pyogenes K56 by temperature-sensitive mutants of the transducing phage A25. Z Naturforsch 24:1556–1561
Primrose SB (1976) Bacterial transduction, patterns of progress. Meadowfield Press Ltd
Schmieger H (1972) Phage P22-mutants with increased or decreased transduction abilities. Mol Gen Genet 119:75–88
Stuttard C (1979) Transduction of auxotrophic markers in a chloramphenicol-producing strain of Streptomyces. J Gen Microbiol 110:479–482
Susskind MM, Botstein D (1978) Molecular genetics of bacteriophage P22. Microbiol Rev 42:385–413
Wall JD, Harriman PD (1974) Phage P1 mutants with altered transducing ability for E. coli. Virology 59:532–544
Walter F, Hartmann M, Klaus S (1981) Restriction endonuclease analysis of DNA from the Streptomyces phages SH3, SH5, SH10, and SH13. Gene 13:57–63
Welsch M (1956) Evidence for the occurrence of true lysogeny among actinomycetes. Virology 2:703–704
Welsch M, (1963) Biology of actinophages. In: Symposium on Genetics and Breeding of Streptomyces. Dubrovnik, Yugoslav Academy of Science and Arts, Zagreb
Zinder ND, Lederberg J (1952) Genetic exchange in Salmonella. J Bacteriol 64:679–699
Zinder ND (1955) Bacterial transduction. J Cell Comp Physiol 45:23–49
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Süss, F., Klaus, S. Transduction in Streptomyces hygroscopicus mediated by the temperate bacteriophage SH10. Molec. Gen. Genet. 181, 552–555 (1981). https://doi.org/10.1007/BF00428752
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00428752