Summary
We isolated an Escherichia coli K-12 regulatory mutation affecting the acetohydroxy acid synthase III isoenzyme. This mutation was found to lie outside the structural genes ilvHI for this isoenzyme and was designated lrs-1. A strain carrying this mutation was found to be altered in the leucine-mediated control of ilvHI mRNA and acetohydroxy acid synthase III synthesis observed in the isogenic lrs + strain. These alterations appeared to be a consequence of a reduced intracellular concentration of a single one of five tRNALeu isoaccepting species.
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Communicated by A. Böck
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Ursini, M.V., Arcari, P. & De Felice, M. Acetohydroxy acid synthase isoenzymes of Escherichia coli K-12: A trans-acting regulatory locus for ilvHI gene expression. Molec. Gen. Genet. 181, 491–496 (1981). https://doi.org/10.1007/BF00428741
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DOI: https://doi.org/10.1007/BF00428741