Abstract
Grass puffer Fugu niphobles, captured in November 1986 at Shimoda, Japan, and then reared at different temperatures ranging from 10 to 29°C, was examined for microflora changes in the skin, gill and intestines. At 10°C, the skin and gill were colonized mainly by Pseudomonas, Flavobacterium and Moraxella, while at 29°C Vibrio and Flavobacterium appeared abundantly. The intestinal microflora, consisting of Vibrio, Pseudomonas and Flavobacterium, revealed little temperature dependence, although the intrageneric composition of Vibrio changed conspicuously depending on the change of water temperature. Vibrio group 1, tentatively identified as V. alginolyticus, was detected in all tissues examined at 20 and 29°C. This, along with the fact that V. alginolyticus produces tetrodotoxin, suggests that the tetrodotoxin contained in puffer is, at least partly, accounted for by this bacterial species inhabiting intestines.
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Literature cited
Anderson, D. P. (1974). Fish Immunology. TFH Publishers, Neptune, New Jersey
Cowan, S. T. (1974). Cowan and Steel's Manual for the Identification of Medical Bacteria. Second ed Cambridge University Press, London
Horsley, R. W. (1977). A review of the bacterial flora of teleosts and elasmobranchs, including methods for its analysis. J. Fish Biol. 10: 529–553
Liston, J. (1957). The occurrence and distribution of bacterial types on flatfish. J. gen. Microbiol. 16: 205–216
Narita, H., Matsubara, S., Miwa, N., Akahane, S., Murakami, M., Goto, T., Nara, M., Noguchi, T., Saito, T., Shida, Y., Hashimoto, K. (1987). Vibrio alginolyticus, a TTX-producing bacterium isolated from the starfish Astropecten polyacanthus. Nippon Suisan Gakk. 53: 617–621
Noguchi, T. (1988). Recent Advances in Tetrodotoxin Research. In: Hashimoto, K. (ed.) Food chain-associated toxification of tetrodotoxin-bearing animals. Koseisha-Koseikaku, Tokyo, p. 85–93
Noguchi, T., Hwang, D. F., Arakawa, O., Sugita, H., Deguchi, Y., Shida, Y., Hashimoto, K. (1987). Vibrio alginolyticus, a tetrodotoxin-producing bacterium, in the intestines of the fish Fugu vermicularis vermicularis. Mar. Biol. 94: 625–630
Sera, H., Ishida, Y., Kadota, H. (1974). Effect of the Ocean Environment on Microbial Activities. In: Colwell, R. R., Morita, R. Y. (eds.) Bacterial flora in the digestive tracts of marine fish. University of Park Press, Baltimore, p. 467–490
Shewan, J. M., Hobbs, G., Hodgkiss, W. (1960). A determinative scheme for the identification of certain genera of Gram-negative bacteria, with special reference to the Pseudomonadaceae. J. appl. Bact. 23: 379–390
Sieburth, J. McN (1967). Seasonal selection of estuarine bacteria by water temperature. J. exp. Mar. Biol. Ecol. 1: 98–121
Starr, M. P., Stolp, H., Trüper, H., Balows, A., Schlegel, H. G. (1981). The Prokaryotes. Springer-Verlag, Berlin
Sugita, H., Noguchi, T., Hwang, D. F., Furuta, M., Motokane, T., Sonoda, T., Hashimoto, K., Deguchi, Y. (1987). Intestinal microflora of coastal puffer fishes. Nippon Suisan Gakk. 53: 2201–2207
Sugita, H., Tanaami, H., Kobashi, T., Deguchi, Y. (1981). Bacterial flora of coastal bivalves. Nippon Suisan Gakk. 47: 655–661
Sugita, H., Ueda, R., Berger, L. R., Deguchi, Y. (1986). A new selective medium for the isolation of anaerobic bacteria from marine animals. Food Microbiol. 3: 289–293
Sugita, H., Ushioka, S., Kihara, D., Deguchi, Y. (1985). Changes in the bacterial composition of water in a carp rearing tank. Aquaculture 44: 243–247
Takahashi, Y., Kajiwaki, T., Itami, T., Okamoto, T. (1987). Enzymatic properties of the bacteriolytic substances in the skin mucus of yellowtail. Nippon Suisan Gakk. 53: 425–431
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Communicated by M. Anraku, Tokyo
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Sugita, H., Iwata, J., Miyajima, C. et al. Changes in microflora of a puffer fish Fugu niphobles, with different water temperatures. Marine Biology 101, 299–304 (1989). https://doi.org/10.1007/BF00428125
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DOI: https://doi.org/10.1007/BF00428125