Abstract
An enzyme that uses GTP as a substrate for the formation of formate and a 2.4.5-triamino-6-hydroxypyrimidine derivative has been determined in the riboflavin overproducing yeast Pichia guilliermondii. In rib 1 mutants this enzyme is absent. This implies an involvement of the enzyme in the riboflavin biosynthesis and indicates that GTP is a purine precursor of riboflavin. Some properties of the GTP cyclohydrolase (substrate specificity, pH and temperature optima, activators and inhibitors, molecular weight, stability) were studied using a partly purified enzyme preparation. Cells grown under riboflavin overproducing conditions (iron deficiency) have 20–40-fold increased enzyme activity as compared with non-overproducing cultures (supplemented with iron).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- RF:
-
riboflavin
- DHRiboyslAP £:
-
2.5-diamino-6-hydroxy-4-(1′-d-ribosylamino)pyrimidine-5′-phosphate
- DHRibitylAP:
-
2.5-diamino-6-hydroxy-4-(1′-ribitylamino)pyrimidine
- neopterin:
-
6-(trihydroxypropyl)pterin
- DMP:
-
6.7-dimethylpterin
- Fe:
-
iron deficient condition
- +Fe:
-
supplemented with iron
References
Bacher, A., Lingens, F.: Biosynthesis of riboflavin. Formation of 6-hydroxy-2.4.5-triaminopyrimidine in rib 7 mutants of Saccharomyces cerevisiae. J. Biol. Chem. 246, 7018–7022 (1971)
Benndorf, R.: Zur Frage der Purinvorstufe für die Riboflavinbiosynthese in der flavinogenen Hefe Candida guilliermondii (Cast.) Lang. et G. Thesis, Fak. Naturwiss., Univ. of Halle (1978)
Burg, A. W., Brown, G. M.: The biosynthesis of folic acid. VIII. Purification and properties of the enzyme that catalyses the production of formate from carbon atom 8 of guanosine triphosphate. J. Biol. Chem. 243, 2349–2358 (1968)
Foor, F., Brown, G. M.: Purification and properties of guanosine triphosphate cyclohydrolase II from Escherichia coli. J. Biol. Chem. 250, 3545–3551 (1975)
Hunger, H.-D., Reinbothe, H.: Säulenchromatographie von 5′-Nucleotiden am Anionenaustauscher Sephadex A-25 mit einem Lineargradieten aus Ammoniumbicarbonat und Ammoniumcarbamat. J. Chromatogr. 97, 273–276 (1974)
Johnson, P. A., Jones-Mortimer, M. C., Quayle, J. R.: Use of a purified bacterial formate dehydrogenase for the microestimation of formate. Biochim. Biophys. Acta 89, 351–353 (1964)
Logvinenko, E. M., Shavlovsky, G. M., Koltun, L. V., Ksheminskaya, H. P.: Precursors of riboflavin in the yeast Pichia guilliermondii. Mikrobiologya (Moscow) 44, 48–54 (1975)
Mailänder, B., Bacher, A.: Biosynthesis of riboflavin. Structure of the purine precursor and origin of the ribityl side chain. J. Biol. Chem. 251, 3623–3628 (1976)
Miersch, J., Logvinenko, E. M., Zakalsky, A. E., Shavlovsky, G. M., Reinbothe, H.: Origin of the ribityl side chain of riboflavin from the ribose moiety of guanosine triphosphate in Pichia guilliermondii yeast. Biochim. Biophys. Acta 543, 305–312 (1978)
Schlee, D.: Bedeutung von GTP-Cyclohydrolasen im Purinstoffwechsel. Pharmazie 31, 448–452 (1977)
Schlee, D., Fritsche, W., Reinbothe, H.: Purinstoffwechsel in Candida guilliermondii (Cast.) Lang. et G. Flora 156, 390–403 (1966)
Schlee, D., Reinbothe, H., Fritsche, W.: Der Einfluß von Eisen auf den Purinstoffwechsel und die Riboflavinbildung von Candida guilliermondii (Cast.) Lang. et G. Z. Allgem. Mikrobiol. 8, 127–138 (1968)
Shavlovsky, G. M., Logvinenko, E. M., Kashchenko, V. E., Koltun, L. V., Zakalsky, A. E.: Determination of GTP cyclohydrolase, the first enzyme of riboflavin biosynthesis from the yeast Pichia guilliermondii. Doklady Akad. Nauk. USSR (Moscow) 230, 1485–1487 (1976a)
Shavlovsky, G. M., Logvinenko, E. M., Trach, V. M.: Changes in activity of riboflavin synthetase at different levels of flavinogenesis in some species of yeast Candida guilliermondii. Doklady Akad. Nauk. USSR (Moscow) 203, 241–244 (1972)
Shavlovsky, G. M., Logvinenko, E. M., Trach, V. M., Senjuta, E. S.: Die Regulation der Synthese der Riboflavinsynthetase durch Eisen in flavinogenen Hefen. Biochem. Physiol. Pflanzen 167, 379–387 (1975)
Shavlovsky, G. M., Chistyakova, V. S.: To the influence of iron on riboflavin (vitamin B2) biosynthesis by the Candida guilliermondii yeast. Doklady Akad. Nauk. USSR (Moscow) 111, 887–889 (1956)
Shavlovsky, G. M., Kashchenko, V. E., Koltun, L. V., Logvinenko, E. M., Zakalsky, A. E.: Regulation of synthesis of GTP cyclohydrolase participating in yeast flavinogenesis by iron. Mikrobiologya (Moscow) 46, 578–580 (1977)
Shavlovsky, G. M., Logvinenko, E. M., Schlee, D., Koltun, L. V.: Regulation of 6-hydroxy-2.4.5-triaminopyrimidine synthesis by riboflavin and iron in riboflavin-deficient mutants of Pichia guilliermondii yeast. Biochim. Biophys. Acta 428, 611–618 (1976b)
Shavlovsky, G. M., Koltun, L. V., Kashchenko, V. E.: Regulation of activity of GTP cyclohydrolase, the enzyme of the first step of flavinogenesis in yeasts. Biochimiya (Moscow) 43, 2074–2081 (1978a)
Shavlovsky, G. M., Sibirny, A. A., Kshanovskaya, B. V., Koltun, L. V.: Genetic classification of riboflavin-deficient mutants of Pichia guilliermondii yeast. Proc. XIV Internat. Genet. Congress, part I, p. 94, Moscow (1978b)
Wolf, W. A., Brown, G. M.: Biosynthesis of folic acid. X. Evidence for an Amadori rearrangement in the enzymatic formation of dihydroneopterin triphosphate from GTP. Biochim. Biophys. Acta 192, 468–478 (1969)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shavlovsky, G.M., Logvinenko, E.M., Benndorf, R. et al. First reaction of riboflavin biosynthesis —Catalysis by a guanosine triphosphate cyclohydrolase from yeast. Arch. Microbiol. 124, 255–259 (1980). https://doi.org/10.1007/BF00427735
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00427735