Summary
A lactate-nitrate medium suitable for genetic studies with nitrate reductase mutants (nar -) of Escherichia coli was devised. This permitted the selection of nar - strains by their failure to use nitrate as terminal electron acceptor during anaerobic growth, in addition to the selection procedure based on the chlorate resistance of nar - mutants. Transduction studies with phage P1 and nar - mutants from both sources demonstrated the existence of at least three nar genes in the gal region of the E. coli linkage map, their relative positions being: gal .... narF .... bio .... narD .... narE. Using λ phage cotransduction of narD with bio was observed and several independently-isolated defective λ-transducing phages were examined. Phage λ also transduced the narF gene with gal linkage but the narE gene was not λ-transducible.
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Venables, W.A., Guest, J.R. Transduction of nitrate reductase loci of Escherichia coli by phages Pl and λ. Molec. Gen. Genet. 103, 127–140 (1968). https://doi.org/10.1007/BF00427140
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DOI: https://doi.org/10.1007/BF00427140