Summary
The fluorescent dye, diamidinophenylindole-dihydrochloride (DAPI) can be added to CsCl gradients to enhance the density resolution of DNA species, independent of their topological configurations. When Proteus mirabilis and Escherichia coli strains carrying an RP4::Mucts plasmid were examined with the use of such a technique, it was found that after thermal induction of the prophage essentially all of the plasmid DNA became associated with the chromosome. This quantitative association is detergent-RNase-and pronase-resistant and dependent on the expression of Mu genes. The association is temporally, and probably functionally, correlated with the onset of Mu DNA replication. Genetic studies with F'::mini Mu plasmids indicate that some of the association results in stable Hfr formation, and does not require the product of Mu gene B.
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References
Allet B (1979) Mu insertion duplicates a 5 base pair sequence at the host inserted site. Cell 16:123–129
Bollum FJ (1966) In: Cantoni GL, Davies DR (eds) Procedures in Nucleic Acid Research. Harper and Row, New York, p 296
Chaconis G, Harshay R, Bukhari A (1980) Association of Mu-containing plasmids with the E. coli chromosome upon prophage induction. Proc Natl Acad Sci USA 77:1778–1782
Clewell DB, Helinski DR (1970) Properties of a supercoiled deoxyribonucleic acid protein complex and strand specificity of the relaxation event. Biochemistry 9:4428–4440
Dénarié J, Rosenberg C, Bergeron B, Boucher C, Michel M, Barate de Bertalino M (1977) Potential of RP4::Mu plasmids for in vivo genetic engineering of Gram negative bacteria. In: Bukhari AI, Shapiro JA, Adhya S (eds) DNA Insertion Elements, Plasmids and Episomes. Cold Spring Harbor Laboratory, New York, p 507
Faelen M, Resibois A, Toussaint A (1978) Mini-Mu: an insertion element derived from temperate phage Mu-1. Cold Spring Harbor Symp Quant Biol 43:1169–1177
Ghosal D, Sommer H, Saedler H (1979) Nucleotide sequence of the transposable DNA-element IS2. Nucleic Acids Res 6:111–1122
Grindley NDF, Sherratt DJ (1978) Sequence Analysis of IS1 Insertion Sites: Models for Transposition. Cold Spring Harbor Symp Quant Biol 43:1257–1260
Kahmann R, Kamp D (1979) Nucleotide sequences of the attachment sites of bacteriophage Mu DNA. Nature 280:247–250
Kuempel P (1972) DNA-DNA hybridization assay for replication origin of Escherichia coli. J Bacteriol 110:917–925
Ljungquist E, Bukhari AI (1977) State of prophage Mu DNA upon induction. Proc Natl Acad Sci USA 74:3143–3147
Miller JH (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Nash HA, Mizuuchi K, Weisberg RA, Kikuchi Y, Gellert M (1977) Integrative recombination of bacteriophage λ — the biochemical approach to DNA insertions. In: Bukhari AI, Shapiro JA, Adhya S (eds) DNA Insertion Elements, Plasmids and Episomes. Cold Spring Harbor Laboratory, New York p 363
O'Day K, Shultz D, Howe M (1978) Search for integration deficient mutants of bacteriophage Mu. Microbiology 1979. Schlessinger D (ed) ASM, Washington DC, p 48
Ohtsubo H, Ohmori H, Ohtsubo E (1978) Nucleotide sequence analysis of Tn3(Ap): implications for insertion and deletion. Cold Spring Harbor Symp Quant Biol 43:1269–1277
Razzaki T, Bukhari AI (1975) Events following prophage Mu induction. J Bacteriol 122:437–442
Reed RR, Young RA, Stutz JA, Grindley NF, Guyer MS (1979) Transposition of the Escherichia coli insertion element γ generates a five base-pair repeat. Proc Natl Acad Sci USA 76:2195–2198
Rosenberg M, Court D, Shimatake H, Brady C, Wulff D (1978) The relationship between function and DNA sequence in an intercistronic regulatory region in phage lambda. Nature 272:414–423
Shapiro JA (1979) Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proc Natl Acad Sci USA 76:1933–1937
Toussaint A, Faclen M, Bukhari AI (1977) Mu-mediated illegitimate recombinations as an integral part of the Mu life cycle. In: Bukhari AI, Shapiro JA, Adhya S (eds) DNA Insertion Elements. Plasmids and Episomes. Cold Spring Harbor Laboratory. New York, p 275
Van Brunt J, Waggoner BT, Pato ML (1977) Re-examination of F Plasmid Replication in a dna C Mutant of Escherichia coli. Mol Gen Genet 150:285–292
van de Putte P, Giphart-Gassler M, Gossen T, Van Meeteren A, Wijffelman C (1978) Is integration essential for Mu replication? In: Hofschneider P, Starlinger P (eds) Integration and Excision of DNA Molecules. Springer, Berlin Heidelberg New York, p 33
van de Putte P, Gruijthuijsen M (1972) Chromosome-mobilization and integration of F-factors in the chromosome of recA strains of e. coli under the influence of bacteriophage Mu-1. Mol Gen Genet 118:173–183
Waggoner BT, Pato ML (1978) Early events in the replication of Mu prophage DNA. J Virol 27:587–594
Wijffelman C, Lotterman B (1977) Kinetics of Mu DNA synthesis. Mol Gen Genet 151:169–174
Williamson DH, Fenell DJ (1974) The Use of Fluorescent DNA-Binding Agent for Detecting and Separating Yeast Mitochondrial DNA. In: Prescott D (ed) Methods in Cell Biology. Vol XII. Academic Press, New York, p 335
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Bialy, H., Waggoner, B.T. & Pato, M.L. Fate of plasmids containing Mu DNA: Chromosome association and mobilization. Molec. Gen. Genet. 180, 377–383 (1980). https://doi.org/10.1007/BF00425851
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DOI: https://doi.org/10.1007/BF00425851