Summary
We have continued the transcriptional analysis of the region of cytological locus 67B that contains the four small heat shock genes and other genes. Transcription from one of the heat shock genes in the region, hsp 26, takes place during high temperature treatment and at certain developmental stages, without heat shock, in several tissues, such as imaginal discs and adult ovaries. Observations of unexpected products after nuclease protection experiments periments provided the first indication of what genomic blot experiments showed to be small deletions. The alleles containing the deletion are expressed at the same level as the wild type allele. The deletion shortens the protein product, implying that it is in the coding region. Furthermore, flies homozygous for one of the deletion alleles are viable.
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References
Ashburner M, Bonner JJ (1979) The induction of gene activity in Drosophila by heat shock. Cell 17:245–254
Bardwell JCA, Craig EA (1984) Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous. Proc Natl Acad Sci USA 84:848–852
Cheney CM, Shearn A (1983) Developmental regulation of Drosophila imaginal disc proteins: Synthesis of a heat shock protein under non-heat shock conditions. Dev Biol 95:325–330
Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991–1995
Corces V, Homgren R, Frenn DR, Morimoto R, Meselson M (1980) Four heat shock protein of Drosophila melanogaster coded within a 12-kilobase region in chromosome subdivision 67B. Proc Natl Acad Sci USA 77:5390–5393
Favaloro J, Treisman R, Kamen R (1980) Transcriptional maps of Polyoma Virus-Specific RNA: analysis by two-dimentinal nuclease S1 gel mapping. In: Grossman L, Moldase K (eds) Methods in enzymology, vol 65. Academic Press, New York, pp 718–799
Henikoff S (1983) Cloning exons for mapping of transcription: characterization of the Drosophila melanogaster alcohol dehydrogenase gene. Nucl Acids Res 11:4735–4752
Hiyashi K (1980) A cloning vehicle suitable for stand separation. Gene 11:109–115
Ingolia TD, Craig EA (1982a) Drosophila gene related to the major heat shock-induced gene is transcribed at normal temperatures and not induced by heat-shock. Proc Natl Acad Sci USA 79:525–529
Ingolia TD, Craig EA (1982b) Four small Drosophila proteins are related to each other and to mammalian α-crystallin. Proc Natl Acad Sci USA 79:2360–2364
Ireland R, Berger E (1982) synthesis of low molecular weight heat shock proteins stimulated by ecdysterone in a culture Drosophila cell line. Proc Natl Acad Sci USA 79:855–859
Laemmli UK (1970) Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lewin B (1983) Genes. John Wiley and Sons, New York, p 325
Maniatis T, Fritsch EF, Sanbrook J (1982) Molecular cloning laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Mason PJ, Hall LMC, Gausz JC (1984) The expression of heat shock genes during normal development in Drosophila melanogaster. Mol Gen Genet 194:73–78
McDonell MW, Simon MN, Studier FW (1977) Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol 110:119–146
Messing J (1983) New M13 vectors for cloning. In: Grossman L, Moldare K (eds) Methods in enzymology, vol 101. Academic Press, New York, pp 20–78
Petersen NS, Moller GM, Mitchell HK (1975) Genetic mapping of the coding regions for three heat-shock proteins in D. melanogaster. Genetics 92:891–902
Poncz M, Solowiejczyk D, Ballantine M, Schwartz E, Surreys S (1982) “Nonrandom DNA” sequence analysis in bacteriophage M13 by the dideoxy chain-termination method. Proc Natl Acad Sci USA 79:4298–4302
Schlesinger MJ, Kelley PM, Aliperti G, Malfer C (1982) In: Schlesinger MJ, Ashburner M, Tissieres A (eds) Heat shock: from bacteria to man. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 243–250
Schneider I (1972) Cell lines derived from flate embryonic stages of Drosophila melanogaster. J Embryol Exp Morph 27:353–365
Sirotkin K (1982) Developmentally regulated transcription of the 67B heat-shock custer. In: Schlesinger MJ, Ashburner M, Tissieres A (eds) Heat shock: from bacteria to man. Cold Spring Harbor, New York, pp 69–75
Sirotkin K, Davidson N (1982) Developmentally regulated transcription from Drosophila melanogaster Chromosomal site 67B. Dev Biol 89:196–210
Southern EM (1975) Detections of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Southgate R, Ayme A, Voellmy R (1983), Nucleotide sequence analysis of the Drosophila small heat shock gene cluster at locus 67B. J Mol Biol 165:35–57
Wadsworth SC (1982) A family of related proteins is encoded for by the major Drosophila of related heat shock gene family. Mol Cell Biol 2:280–292
Zimmerman JL, Petri W, Meselson M (1983) Accumulation of a specific subset of DM heat shock mRNAs in normal development without heat shock. Cell 32:1161–1170
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Sirotkin, K., Bartley, N., Perry, W.L. et al. Deletion polymorphism in a Drosophila melanogaster heat shock gene. Molec. Gen. Genet. 204, 266–272 (1986). https://doi.org/10.1007/BF00425508
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DOI: https://doi.org/10.1007/BF00425508