Summary
A survey was made of various visible light absorption spectra of whole cells, particulate and soluble fractions and haem extracts of representative strains of all known species of sulphate-reducing bacteria. The previously accepted distinction that Desulfovibrio species contain only a c-type cytochrome whereas Desulfotomaculum species contain only a b-type cytochrome was not confirmed. The pigment contents of the genera Desulfovibrio and Desulfotomaculum were not completely distinct from each other, but both genera had characteristic spectral patterns. Reduced minus oxidized spectra of whole cells and particulate fractions showed the presence of b-type cytochromes in all Desulfotomaculum species and in Desulfovibrio africanus. However, protohaem, the prosthetic group of b-type cytochromes, occurred in haem extracts from all species, although only just detectable in the extract from Desulfovibrio vulgaris NCIB 8303. Particulate c-type cytochromes were found in Desulfotomaculum orientis, Desulfotomaculum nigrificans and all the Desulfovibrio species, but the amount in Desulfotomaculum nigrificans was very small. Only the Desulfovibrio species contained soluble c-type cytochromes. Spectral properties indicated that a d-type cytochrome might exist in species in addition to Desulfovibrio africanus, but no supporting evidence was obtained from results of haem extractions. Some spectra contained peaks which could not be identified.
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References
Barrett, J.: The prosthetie group of cytochrome a 2. Biochem. J. 64, 626–639 (1956).
Campbell, L. L., Postgate, J. R.: Classification of the sporeforming sulfate-reducing bacteria. Bact. Rev. 29, 359–363 (1965).
Drucker, H., Trousil, E. B., Campbell, L. L.: Purification and properties of cytochrome c 3 of Desulfovibrio salexigens. Biochemistry 9, 3395–3400 (1970).
Florkin, M., Stotz, F. H. (Eds.): Comprehensive biochemistry, 2nd ed., vol. 13, pp. 18–24. Amsterdam: Elsevier 1965.
Ishimoto, M., Koyama, J., Nagai, Y.: Biochemical studies on sulfate-reducing bacteria. IV. The cytochrome system of sulfate-reducing bacteria. J. Biochem. (Tokyo) 41, 763–770 (1954).
Itzhaki, R. F., Gill, D. M.: A micro-biuret method for estimating proteins. Analyt. Biochem. 9, 401–410 (1964).
Jones, H. E.: Sulfate-reducing bacterium with unusual morphology and pigment content. J. Bact. 106, 339–346 (1971a).
—: A re-examination of Desulfovibrio africanus. Arch. Mikrobiol. 80, 78–86 (1971b).
Kielin, D.: Precautions in the use of ferri-or ferrocyanide for the study of haemoproteins. Nature (Lond.) 190, 717–718 (1961).
Le Gall, J., Bruschi-Heriaud, M.: Purification and some properties of a new cytochrome from Desulfovibrio desulfuricans. In: Symposium: Structure and function of cytochromes, pp. 467–470. Ed. by K. Okunuki, M. D. Kamen, I. Sekuzu. Tokyo: University Tokyo Press; Baltimore, Md.: University Park Press 1968.
——, Devartanian, D. V.: Electron paramagnetic resonance and light absorption studies on c-type cytochromes of the anaerobic sulfate reducer Desulfovibrio. Biochim. biophys. Acta (Amst.) 234, 499–512 (1971).
Miller, J. D. A., Saleh, A. M.: A sulphate-reducing bacterium containing cytochrome c3 but lacking desulfoviridin. J. gen. Microbiol. 37, 419–423 (1964).
Porra, R. J., Vitols, K. S., Labbe, R. F., Newton, N. A.: Studies on ferrochelatase. The effects of thiols and other factors on the determination of activity. Biochem. J. 104, 321–327 (1967).
Postgate, J. R.: On the nutrition of Desulfovibrio desulfuricans. J. gen. Microbiol. 5, 714–724 (1951).
—: Cytochrome c 3 and desulfoviridin; pigments of the anaerobe Desulfovibrio desulfuricans. J. gen. Microbiol. 14, 545–572 (1956).
—, Campbell, L. L.: Classification of Desulfovibrio species, the non-sporulating sulfate-reducing bacteria. Bact. Rev. 30, 732–738 (1966).
Trudinger, P. A.: Carbon monoxide reacting pigment from Desulfotomaculum nigrificans and its possible relevance to sulfite reduction. J. Bact. 104, 158–170 (1970).
Yagi, T.: Formate: cytochrome oxidoreductase of Desulfovibrio vulgaris. J. Biochem. (Tokyo) 66, 473–478 (1969).
—, Maruyama, K.: Purification and properties of cytochrome c3 of Desulfovibrio vulgaris, Miyazaki. Biochim. biophys. Acta (Amst.) 243, 214–224 (1971).
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Jones, H.E. Cytochromes and other pigments of dissimilatory sulphate-reducing bacteria. Archiv. Mikrobiol. 84, 207–224 (1972). https://doi.org/10.1007/BF00425199
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DOI: https://doi.org/10.1007/BF00425199