Summary
The preparation of ribosomal particles from P. chrysogenum has been described. After correction to 20°C and extrapolation to infinite dilution, they had sedimentation coefficients of approximately 80S, 60 S, and 40 S. The washed ribosomes plus purified supernatant fraction, KCl and sucrose incorporated C14-l-amino acids into protein. There was no stimulation of incorporation by ATP, GTP, CTP, UTP, or Mg ions. Incorporation was inhibited by streptomycin and chloramphenicol but not by ribonuclease.
The amino acid incorporating system from P. chrysogenum did not resemble bacterial and yeast systems in many respects and reasons for this are discussed.
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Albaum, H. G., and W. W. Umbreit: Differentiation between ribose-3-phosphate and ribose-5-phosphate by means of the orcinol-pentose reaction. J. biol. Chem. 167, 369–376 (1947).
Berg, P.: Acyl adenylates: The interaction of adenosine triphosphate and l-methionine. J. biol. Chem. 222, 1025–1034 (1956).
—, F. H. Bergmann, E. J. Ofengand, and M. Dieckmann: The enzymatic synthesis of amino acyl derivatives of ribonucleic acid. I. The mechanism of leucylvalyl-, isoleucyl-, and methionyl ribonucleic acid formation. J. biol. Chem. 236, 1726–1734 (1961).
Bracker, C. E., and E. E. Butler: The ultrastructure and development of septa in hyphae of Rhizoctonia solani. Mycologia (N. Y.) 55, 35–58 (1963).
Bretthauer R. K., L. Marcus, J. Chaloupka, H. O. Halvorson, and R. M. Bock: Amino acid incorporation into protein by cell-free extracts of yeast. Biochemistry 2, 1079–1084 (1963).
De Moss, J. A., and G. D. Novelli: An amino acid dependent exchange between 32P labelled inorganic pyrophosphate and ATP in microbial extracts. Biochim. biophys. Acta (Amst.) 22, 49–61 (1956).
Gale, Ernest F.: The synthesis of proteins and nucleic acids. The Bacteria, III, p. 471–576 (Ed. Gunsalus and Stanier). New York: Academic Press 1962.
Gillchriest, W. C., and R. M. Bock: Isolation and characterization of bacterial nucleoprotein particles, p. 1–10. In. R. B. Roberts (ed.) Microsomal particles and protein synthesis. New York: Pergamon Press 1963.
Greene, H. C., and E. B. Fred: Maintenance of vigorous mold stock cultures. Ind. Eng. Chem. 26, 1297–1299 (1934).
Haidle, Charles W.: Incorporation of amino acids into protein by cell-free extracts of Penicillium chrysogenum. Ph. D. Dissertation. Madison, Wisconsin: University of Wisconsin 1964.
Hoagland, M. B.: An enzymic mechanism for amino acid activation in animal tissue. Biochim. biophys. Acta (Amst.) 16 288–289 (1955).
—, E. B. Keller, and P. C. Zamecnik: Enzymatic carboxyl activation of amino acids. J. biol. Chem. 218, 345–358 (1956).
—, P. C. Zamnecnik, and M. L. Stephenson: Intermediate reactions in protein biosynthesis Biochim. biophys. Acta (Amst.) 24, 215–216 (1957).
Kornfeld, J. M.: Structural and physiological aspects of germination of conidia of Penicillium chrysogenum. Ph. D. Dissertation. Madison, Wisconsin: University of Wisconsin 1961.
Littlefield, J. W., E. B. Keller, J. Gross, and P. C. Zamecnik: Studies on cytoplasmic ribonucleoprotein particles from the liver of the rat. J. biol. Chem. 217, 111–123 (1955).
McQuillen, K., R. B. Roberts, and R. J. Britten: Synthesis of nascent protein by ribosomes in E. coli. Proc. nat. Acad. Sci. (Wash.) 45, 1437–1447 (1959).
Marcus, L., R. K. Bretthauer, R. M. Bock, and H. O. Halvorson: The effect of poly U size on the incorporation of phenylalanine in the cell-free yeast system. Proc. nat. Acad. Sci. (Wash.) 50, 782–789 (1963).
Matthaei, J. H., and M. W. Nirenberg: Characteristics and stabilization of DNAase-sensitive protein synthesis in E. coli extracts. Proc. nat. Acad. Sci. (Wash.) 47, 1580–1588 (1961).
Mazzone, H. M. Biophysical and biochemical studies of squash mosaic virus and related macromolecules. Ph. D. Dissertation. Madison, Wisconsin: University of Wisconsin 1959.
Schachman, H. K.: Ultracentrifugation in biochemistry. New York: Academic Press, Inc. 1959.
Shatkin, A. J., and E. L. Tatum: Electron microscopy of Neurospora crassa mycelia. J. biophys. biochem. Cytol. 6, 423–426 (1959).
Simpson, M. V.: Protein biosynthesis. Ann. Rev. Biochem. 31, 333–368 (1962).
Storck, R.: Characterization of ribosomes from Neurospora crassa. Biophys. J. 3, 1–10 (1963).
Uemura I., K. Okuda and T. Winnick Biosynthesis of gramicidins and tyrocidins in cell-free preparations from Bacillus brevis. Biochemistry 2, 719–725 (1963).
Wainwright, S. D.: On the formation of tryptophan synthetase enzyme by cell-free extracts of mycelium of Neurospora crassa. Canad. J. Biochem. 41, 1327–1329 (1963).
—, and E. S. McFarlane: Partial purification of the “messenger RNA” of Neurospora crassa controlling the formation of tryptophan synthetase enzyme. Biochembiophys. Res. Commun. 9, 529–533 (1962).
Yin, F. H., and R. M. Bock Physical and chemical studies on the proteins of yeast ribosomes. Fed. Proc. 19, 137 (1960).
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Haidle, C.W., Kornfeld, J.M. & Knight, S.G. Incorporation of amino acids into protein by cell-free extracts of penicillium chrysogenum. Archiv. Mikrobiol. 53, 41–49 (1966). https://doi.org/10.1007/BF00424738
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DOI: https://doi.org/10.1007/BF00424738