Abstract
Poly(A)-containing 9S RNA from chick reticulocytes was electrophoresed on formamide-polyacrylamide gels. The molecular weight was determined to be 211 000±10 000 daltons. The RNA was separated into three different fractions with respect to molecular weight. These RNAs were translated in a wheat germ cell-free system. The lower molecular weight RNA directed up to 95% α-chain synthesis, compared to 60% for the higher molecular weight RNA. This was accompanied by a relative increase for β-chain synthesis with increasing molecular weight. It could also be shown by hybridization with labelled poly(U) that the average poly(A) length decreased from about 83 nucleotides for fraction I to 36 nucleotides for fraction III. Our results suggest that fractionation of avian 9 S globin mRNA by electrophoresis on formamide-polyacrylamide gels is dependent upon two parameters, namely differences in the lengths of the non-poly(A)-containing portion of the α and β mRNAs and differences in the poly(A) lengths.
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Morrison, M. R., Brinkley, S. A., Gorski, J., and Lingrel, J. B., J. Biol. Chem. 249, 5290 (1974).
Kazazian, H. H., Snyder, P. G., and Cheng, T., Biochem. Biophys. Res. Comm. 59, 1053 (1974).
Berns, A., Jansen, P., and Bloemendal, H., FEBS Letters 47, 343 (1974).
Hamlyn, P. H. and Gould, H. J., J. Mol. Biol. 94, 101 (1975).
Forget, B. G., Housman, D., Benz, E. J., and McCaffrey, R. P., Proc. Nat. Acad. Sci. U.S.A. 72, 984 (1975).
Moss, B. A. and Hamilton, E. A., Biochim. Biophys. Acta 37, 379 (1974).
Knöchel, W., Hendrick, D., Schröter, S., and Tiedemann, H., Hoppe-Seyler's Z. Physiol. Chem. 354, 1389 (1973).
Wu, R. S. and Wilt, F. H., Develop. Biol. 41, 352 (1974).
Staynov, D. Z., Pinder, J. C., and Gratzer, W. B. Nat. New Biol. 235, 108 (1972).
Cann, A., Gambino, R., Banks, J., and Bank, A., J. Biol. Chem. 249, 7536 (1974).
Roberts, B. E. and Paterson, B. M., Proc. Nat. Acad. Sci. U.S.A. 70, 2330 (1973).
Hendrick, D., Knöchel, W., Schwarz, W., Pitzel, S., and Tiedemann, H., Develop. Biol. 36, 299 (1974).
Shapira, G., Rosa, J., Maleknia, N., and Padieu, P., Methods Enzymol. 12 B, 749 (1968).
Lockard, R. E. and Lingrel, J. B., Nat. New Biol. 233, 204 (1971).
Rektorys, K., Survey of Applicable Mathematics, The MIT Press, Cambridge, Mass. (U.S.A.) 1969, pp. 1273–1274.
Aviv, H. and Leder, P., Proc. Nat. Acad. Sci. U.S.A. 69, 1408 (1972).
Kirschenbaum, D. M., Anal. Biochem. 66, 590 (1975).
Lockard, R. E. and Lingrel, J. B., J. Biol. Chem. 247, 4174 (1972).
Sampson, J. and Borghetti, A. F., Nat. New. Biol. 238, 200 (1972).
Housman, D., Pemberton, R., and Taber, R., Proc. Nat. Acad. Sci. U.S.A. 68, 2716 (1971).
Matsuda, G., Takei, H., Wu, K. C., and Shiozawa, T., Int. J. Protein Res. 3, 173 (1971).
Matsuda, G., Maita, T., Mizuno, K., and Ota, H., Nat. New Biol. 244, 244 (1973).
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Knochel, W., Lange, D. & Hendrick, D. Molecular weights, poly(A)-content, and partial separation of chick globin mRNAs. Mol Biol Rep 3, 143–150 (1976). https://doi.org/10.1007/BF00423228
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DOI: https://doi.org/10.1007/BF00423228