Abstract
A Sephadex gel filtration technique was compared with a radial immunodiffusion (RID) method for the assay of serum haptoglobin in the dog. Administration for a haemolytic agent (acetylphenylhydrazine) to dogs resulted in reduced serum haptoglobin but without a significant change in red cell indices. It is suggested that the assay of serum haptoglobin in the dog may be of value in detecting low grade haemolysis when other criteria fail. After standardising the assay using the gel filtration technique, a number of samples can be screened simultaneously using RID thus enabling routine clinical laboratory monitoring for haemolysis. The clinical value of serum haptoglobin assay in the diagnosis of canine haemolytic anaemia is discussed.
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Jones, D.R.E., Hall, D.E. The assay of canine serum haptoglobin and its diagnostic implications. A comparison of two assay techniques. Comparative Haematology International 1, 29–34 (1991). https://doi.org/10.1007/BF00422690
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DOI: https://doi.org/10.1007/BF00422690