Summary
Curing of the viruslike particles harbored by a strain of the yeast Yarrowia lipolytica was achieved by UV irradiation. The cured strain was found to be able to maintain the viruslike particles after their re-introduction by crossing or by cytoplasmic fusion. The involvement of a UV-induced mutation of a yeast maintenance gene seems therefore unlikely.
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Abbreviations
- UV :
-
ultraviolet
- dsRNA :
-
double-stranded RNA
- VLPs :
-
viruslike particles, A and B alleles of the mating type locus
- TCA :
-
trichloracetic acid
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Tréton, B.Y., Le Dall, M.T. & Heslot, H. UV-induced curing of the double-stranded RNA virus of the yeast Yarrowia lipolytica . Curr Genet 12, 37–39 (1987). https://doi.org/10.1007/BF00420725
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DOI: https://doi.org/10.1007/BF00420725