Summary
Mitochondrial (mt) DNA of the alkane yeast, Saccharomycopsis lipolytica, was isolated. Its buoyant density in CsCl was found to be of 1.687 g/cm3, indicating a GC content of 27.5% and its melting point Tm = 79.5 °C, indicating a GC content of 24.9%. The corresponding values for nuclear (n) DNA, are 1.709 g/cm3 (GC: 49.5%) and Tm = 90.5 (GC: 51.7%) respectively. Electron microscopy revealed that mtDNA has a circular structure with a contour length of about 14.5 µm corresponding to 45.5 kb per molecule. The size estimated from restriction analyses performed with 7 endonucleases was 48.35 kb/molecule. A restriction map was constructed, using the cleavage data of 4 endonucleases.
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References
Arrand JB, Myers PA, Roberts RJ (1978) J Mol Biol 188:127–135
Bak AL, Christiansen C, Stenderup A (1969) Nature 224:270–271
Bernardi G (1975) Molecular genetics of yeast mitochondria. In: Bernardi G, Gros F (eds) Organization and expression of the eukaryotec genome. Biochemical mechanism of differentiation in prokaryotes and eukaryotes. North-Holland/American Elsevier New York
Cryer DR, Eccleshall R, Marmur J (1975) Isolation of yeast DNA. In: Prescott DM (ed) Methods in cell biology, Academic Press, New York, p 39
Davis RW, Simon M, Davidson N (1971) Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 21. Academic Press, New York p 413
Del Giudice L, Wolf K, Sassone-Corsi P, Alvino C (1978) Mol Gen Genet 164:289–293
Esser K, Stahl U (1976) Mol Gen Genet 146:101–106
Esser K, Tudzynski P, Stahl U, Kück U (1980) Mol Gen Genet 178:213–216
Ferenczy L, Maraz A (1977) Nature 268:524–525
Gaillardin CM, Charoy V, Heslot H (1973) Arch Mikrobiol 92:69–83
Gillham NW (1978) Organelle heredity. Raven Press New York
Gunge N, Sakaguchi K (1979) Mol Gen Genet 170:243–247
Hollenberg CP, Borst P, van Bruggen EFJ (1970) Biochim Biophys Acta 209:1–15
Humphreys GO, Willshaw GA, Anderson ES (1975) Biochim Biophys Acta 383:457–463
Knecht R, Präve P, Seipenbusch R, Sukatsch DA (1977) Process Biochemistry 12:4–9
Lang D, Mitani M (1970) Biopolymers 9:373–379
Marmur S, Doty P (1962) J Mol Biol 5:109–118
McDonell MW, Simon MN, Studier FW (1977) J Mol Biol 110:119–149
Meyers JA, Sanchez D, Elwell LP, Falkow S (1976) J Bacteriol 127:1529–1537
Murray K, Murray NE (1975) J Mol Biol 98:551–564
Palmer ML, Raker MA, Kennedy PJ, Young JW, Barnes WM, Rodrigez RL, Noller HF (1979) Mol Gen Genet 172:171–178
Stahl U, Esser K (1979) Eur J Appl Mikrobiol Biotechnol 8:271–278
Stahl U, Kück U, Tudzynski P, Esser K (1980) Mol Gen Genet 178:639–646
Stüber D, Bujard H (1977) Mol Gen Genet 154:299–303
Szybalski W (1968) Use of cesium sulfate for equilibrium density gradient centrifugation. In: Colowick SP, Kaplan NO (eds) Methods in Enzymology, vol 12, Academic Press, New York p 330
Tabak HF, Weijers PJ (1976) Febs Lett 69/1:211–215
Thuring RWJ, Sanders JPM, Borst P (1975) Anal Biochem 66:213–220
Tudzynski P, Esser K (1979) Mol Gen Genet 173:71–84
Wickerham LJ, Kurtzman CP, Herman AI (1969) Sexuality in Candida lipolytica. In: Recent trends in yeast research. Miner Institute, New York
Wickerham LJ, Kurtzman CP, Herman AI (1970) Science 167:1141
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Kück, U., Stahl, U., Lhermitte, A. et al. Isolation and characterization of mitochondrial DNA from the alkane yeast Saccharomycopsis lipolytica . Curr Genet 2, 97–101 (1980). https://doi.org/10.1007/BF00420620
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DOI: https://doi.org/10.1007/BF00420620