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Mitochondrial Genomics and Proteomics of Yarrowia lipolytica

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Yarrowia lipolytica

Part of the book series: Microbiology Monographs ((MICROMONO,volume 24))

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Abstract

In recent years, we have developed the obligate aerobic yeast Y. lipolytica as a model system to analyse respiratory chain complex I (proton pumping NADH:ubiquinone oxidoreductase). Our studies were aimed at exploring structural and functional constraints for the reaction mechanism at the catalytic core of this giant enzyme, defined as the site where ubiquinone reduction couples to proton translocation. Extensive genomic and proteomic analyses of Y. lipolytica mitochondria resulted in the discovery of 41 different complex I subunits, encoded both by the mitochondrial and the nuclear genome. Most of the subunits of complex V (ATP synthase) and a protein specifically involved in complex I iron–sulfur cluster assembly were also described. Novel gel-electrophoretic separation techniques and analytical methods, especially laser-induced liquid bead ion desorption (LILBID) mass spectrometry, developed for these purposes, have great potential as useful tools for obtaining molecular fingerprints of large protein assemblies.

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References

  • Abdrakhmanova A, Zickermann V, Bostina M, Radermacher M, Schägger H, Kerscher S, Brandt U (2004) Subunit composition of mitochondrial complex I from the yeast Yarrowia lipolytica. Biochim Biophys Acta 1658:148–156

    Article  PubMed  CAS  Google Scholar 

  • Abdrakhmanova A, Dobrynin K, Zwicker K, Kerscher S, Brandt U (2005) Functional sulfurtransferase is associated with mitochondrial complex I from Yarrowia lipolytica, but is not required for assembly of its iron-sulfur clusters. FEBS Lett 579:6781–6785

    Article  PubMed  CAS  Google Scholar 

  • Abdrakhmanova A, Zwicker K, Kerscher S, Zickermann V, Brandt U (2006) Tight binding of NADPH to the 39-kDa subunit of complex I is not required for catalytic activity but stabilizes the multiprotein complex. Biochim Biophys Acta 1757:1676–1682

    Article  PubMed  CAS  Google Scholar 

  • Ahlers P, Garofano A, Kerscher S, Brandt U (2000a) Application of the obligate aerobic yeast Yarrowia lipolytica as a eukaryotic model to analyze Leigh Syndrome mutations in the complex I core subunits PSST and TYKY. Biochim Biophys Acta 1459:258–265

    Article  PubMed  CAS  Google Scholar 

  • Ahlers P, Zwicker K, Kerscher S, Brandt U (2000b) Function of conserved acidic residues in the PSST-homologue of complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica. J Biol Chem 275:23577–23582

    Article  PubMed  CAS  Google Scholar 

  • Arthur H, Watson K (1976) Thermal adaptation in yeasts: growth temperatures, membrane lipid, and cytochrome composition of psychrophilic, mesophilic, and thermophilic yeasts. J Bacteriol 128:56–68

    PubMed  CAS  Google Scholar 

  • Baradaran R, Berrisford JM, Minhas GS, Sazanov LA (2013) Crystal structure of the entire respiratory complex I. Nature 494:443–448

    Article  PubMed  CAS  Google Scholar 

  • Barth G, Gaillardin C (1996) Yarrowia lipolytica. In: Wolf K (ed) Non-conventional yeasts in biotechnology. Springer, Heidelberg, pp 313–388

    Chapter  Google Scholar 

  • Bordo D, Bork P (2002) The rhodanese/Cdc25 phosphatase superfamily – sequence-structure-function relations. EMBO Rep 3:741–746

    Article  PubMed  CAS  Google Scholar 

  • Brandt U (2006) Energy converting NADH: quinone oxidoreductase (complex I). Annu Rev Biochem 75:69–92

    Article  PubMed  CAS  Google Scholar 

  • Brandt U, Abdrakhmanova A, Zickermann V, Galkin A, Dröse S, Zwicker K, Kerscher S (2005) Structure–function relationships in mitochondrial complex I of the strictly aerobic yeast Yarrowia lipolytica. Biochem Soc Trans 33:840–844

    Article  PubMed  CAS  Google Scholar 

  • Brockmann C, Diehl A, Rehbein K, Strauss H, Schmieder P, Korn B, Kuhne R, Oschkinat H (2004) The oxidized subunit B8 from human complex I adopts a thioredoxin fold. Structure 12:1645–1654

    Article  PubMed  CAS  Google Scholar 

  • Bych K, Kerscher S, Netz DJ, Pierik AJ, Zwicker K, Huynen MA, Lill R, Brandt U, Balk J (2008) The iron-sulphur protein Ind1 is required for effective complex I assembly. EMBO J 27:1736–1746

    Article  PubMed  CAS  Google Scholar 

  • Carroll J, Shannon RJ, Fearnley IM, Walker JE, Hirst J (2002) Definition of the nuclear encoded protein composition of bovine heart mitochondrial complex I – identification of two new subunits. J Biol Chem 277:50311–50317

    Article  PubMed  CAS  Google Scholar 

  • Carroll J, Fearnley IM, Skehel JM, Shannon RJ, Hirst J, Walker JE (2006) Bovine complex I is a complex of 45 different subunits. J Biol Chem 281:32724–32727

    Article  PubMed  CAS  Google Scholar 

  • Clason T, Zickermann V, Ruiz T, Brandt U, Radermacher M (2007) Direct localization of the 51 and 24 kDa subunits of mitochondrial complex I by three-dimensional difference imaging. J Struct Biol 159:433–442

    Article  PubMed  CAS  Google Scholar 

  • Clason T, Ruiz T, Schagger H, Peng G, Zickermann V, Brandt U, Michel H, Radermacher M (2010) The structure of eukaryotic and prokaryotic complex I. J Struct Biol 169:81–88

    Article  PubMed  CAS  Google Scholar 

  • Cronan JE, Fearnley IM, Walker JE (2005) Mammalian mitochondria contain a soluble acyl carrier protein. FEBS Lett 579:4892–4896

    Article  PubMed  CAS  Google Scholar 

  • Degli Esposti M (1998) Inhibitors of NADH-ubiquinone reductase: an overview. Biochim Biophys Acta 1364:222–235

    Article  PubMed  CAS  Google Scholar 

  • Djafarzadeh R, Kerscher S, Zwicker K, Radermacher M, Lindahl M, Schägger H, Brandt U (2000) Biophysical and structural characterization of proton-translocating NADH-dehydrogenase (complex I) from the strictly aerobic yeast Yarrowia lipolytica. Biochim Biophys Acta 1459:230–238

    Article  PubMed  CAS  Google Scholar 

  • Dobrynin K, Abdrakhmanova A, Richers S, Hunte C, Kerscher S, Brandt U (2010) Characterization of two different acyl carrier proteins in complex I from Yarrowia lipolytica. Biochim Biophys Acta 1797:152–159

    Article  PubMed  CAS  Google Scholar 

  • Dujon B, Sherman D, Fischer G, Durrens P, Casaregola S, Lafontaine I, de Montigny J, Marck C, Neuveglise C, Talla E, Goffard N, Frangeul L, Aigle M, Anthouard V, Babour A, Barbe V, Barnay S, Blanchin S, Beckerich JM, Beyne E, Bleykasten C, Boisrame A, Boyer J, Cattolico L, Confanioleri F, De Daruvar A, Despons L, Fabre E, Fairhead C, Ferry-Dumazet H, Groppi A, Hantraye F, Hennequin C, Jauniaux N, Joyet P, Kachouri R, Kerrest A, Koszul R, Lemaire M, Lesur I, Ma L, Muller H, Nicaud JM, Nikolski M, Oztas S, Ozier-Kalegeropoulos O, Pellenz S, Potier S, Richard GF, Straub ML, Suleau A, Swennen D, Tekaia F, Wesolowski-Louvel M, Westhof E, Wirth B, Zeniou-Meyer M, Zivanovic I, Bolotin-Fukuhara M, Thierry A, Bouchier C, Caudron B, Scarpelli C, Gaillardin C, Weissenbach J, Wincker P, Souciet JL (2004) Genome evolution in yeasts. Nature 430:35–44

    Article  PubMed  Google Scholar 

  • Efremov RG, Baradaran R, Sazanov LA (2010) The architecture of respiratory complex I. Nature 465:441–445

    Article  PubMed  CAS  Google Scholar 

  • Fendel U, Tocilescu MA, Kerscher S, Brandt U (2008) Exploring the inhibitor binding pocket of respiratory complex I. Biochim Biophys Acta 1777:660–665

    Article  PubMed  CAS  Google Scholar 

  • Friedrich T (1998) The NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli. Biochim Biophys Acta 1364:134–146

    Article  PubMed  CAS  Google Scholar 

  • Friedrich T, Ohnishi T, Forche E, Kunze B, Jansen R, Trowitzsch W, Hofle G, Reichenbach HX, Weiss H (1994) Two binding sites for naturally occurring inhibitors in mitochondrial and bacterial NADH:ubiquinone oxidoreductase (complex I). Biochem Soc Trans 22:226–230

    PubMed  CAS  Google Scholar 

  • Galkin AS, Grivennikova VG, Vinogradov AD (2001) H+/2e- stoichiometry of the NADH:ubiquinone reductase reaction catalyzed by submitochondrial particles. Biochemistry (Mosc) 66:435–443

    Article  CAS  Google Scholar 

  • Galkin A, Dröse S, Brandt U (2006) The proton pumping stoichiometry of purified mitochondrial complex I reconstituted into proteoliposomes. Biochim Biophys Acta 1757:1575–1581

    Article  PubMed  CAS  Google Scholar 

  • Garofano A, Zwicker K, Kerscher S, Okun P, Brandt U (2003) Two aspartic acid residues in the PSST-homologous NUKM subunit of complex I from Yarrowia lipolytica are essential for catalytic activity. J Biol Chem 278:42435–42440

    Article  PubMed  CAS  Google Scholar 

  • Grgic L, Zwicker K, Kashani-Poor N, Kerscher S, Brandt U (2004) Functional significance of conserved histidines and arginines in the 49 kDa subunit of mitochondrial complex I. J Biol Chem 279:21193–21199

    Article  PubMed  CAS  Google Scholar 

  • Hiltunen JK, Okubo F, Kursu VAS, Autio KJ, Kastaniotis AJ (2005) Mitochondrial fatty acid synthesis and maintenance of respiratory competent mitochondria in yeast. Biochem Soc Trans 33:1162–1165

    Article  PubMed  CAS  Google Scholar 

  • Hiltunen JK, Schonauer MS, Kaija JA, Mittelmeier TM, Kastaniotis AJ, Dieckmann CL (2008) Mitochondrial fatty acid synthesis type II: more than just fatty acids. J Biol Chem 284:9011–9015

    Article  PubMed  Google Scholar 

  • Hofhaus G, Weiss H, Leonard K (1991) Electron microscopic analysis of the peripheral and membrane parts of mitochondrial NADH dehydrogenase (complex I). J Mol Biol 221:1027–1043

    Article  PubMed  CAS  Google Scholar 

  • Huang GC, Lu H, Hao AJ, Ng DCH, Ponniah S, Guo K, Lufei CC, Zeng Q, Caoj XM (2004) GRIM-19, a cell death regulatory protein, is essential for assembly and function of mitochondrial complex I. Mol Cell Biol 24:8447–8456

    Article  PubMed  CAS  Google Scholar 

  • Hunte C, Zickermann V, Brandt U (2010) Functional modules and structural basis of conformational coupling in mitochondrial complex I. Science 329:448–451

    Article  PubMed  CAS  Google Scholar 

  • Joseph-Horne T, Hollomon DW, Wood PM (2001) Fungal respiration: a fusion of standard and alternative components. Biochim Biophys Acta 1504:179–195

    Article  PubMed  CAS  Google Scholar 

  • Kashani-Poor N, Kerscher S, Zickermann V, Brandt U (2001a) Efficient large scale purification of his-tagged proton translocating NADH : ubiquinone oxidoreductase (complex I) from the strictly aerobic yeast Yarrowia lipolytica. Biochim Biophys Acta 1504:363–370

    Article  PubMed  CAS  Google Scholar 

  • Kashani-Poor N, Zwicker K, Kerscher S, Brandt U (2001b) A central functional role for the 49-kDa subunit within the catalytic core of mitochondrial complex I. J Biol Chem 276:24082–24087

    Article  PubMed  CAS  Google Scholar 

  • Kerscher S (2000) Diversity and origin of alternative NADH:ubiquinone oxidoreductases. Biochim Biophys Acta 1459:274–283

    Article  PubMed  CAS  Google Scholar 

  • Kerscher S, Okun JG, Brandt U (1999) A single external enzyme confers alternative NADH:ubiquinone oxidoreductase activity in Yarrowia lipolytica. J Cell Sci 112:2347–2354

    PubMed  CAS  Google Scholar 

  • Kerscher S, Durstewitz G, Casaregola S, Gaillardin C, Brandt U (2001a) The complete mitochondrial genome of Yarrowia lipolytica. Comp Funct Genomics 2:80–90

    Article  PubMed  CAS  Google Scholar 

  • Kerscher S, Eschemann A, Okun PM, Brandt U (2001b) External alternative NADH:ubiquinone oxidoreductase redirected to the internal face of the mitochondrial inner membrane rescues complex I deficiency in Yarrowia lipolytica. J Cell Sci 114:3915–3921

    PubMed  CAS  Google Scholar 

  • Kerscher S, Kashani-Poor N, Zwicker K, Zickermann V, Brandt U (2001c) Exploring the catalytic core of complex I by Yarrowia lipolytica yeast genetics. J Bioenerg Biomembr 33:187–196

    Article  PubMed  CAS  Google Scholar 

  • Kerscher S, Grgic L, Garofano A, Brandt U (2004) Application of the yeast Yarrowia lipolytica as a model to analyse human pathogenic mutations in mitochondrial complex I. Biochim Biophys Acta 1659:197–205

    Article  PubMed  CAS  Google Scholar 

  • Kurtzman CP (1994) Molecular taxonomy of the yeasts. Yeast 10:1727–1740

    Article  PubMed  CAS  Google Scholar 

  • Lagunas R (1986) Misconceptions about the energy metabolism of Saccharomyces cerevisiae. Yeast 2:221–228

    Article  PubMed  CAS  Google Scholar 

  • Lazarou M, Thorburn DR, Ryan MT, McKenzie M (2009) Assembly of mitochondrial complex I and defects in disease. Biochim Biophys Acta 1793:78–88

    Article  PubMed  CAS  Google Scholar 

  • Lufei C, Ma J, Huang G, Zhang T, Novotny-Diermayr V, Ong CT, Cao X (2003) GRIM-19, a death-regulatory gene product, suppresses Stat3 activity via functional interaction. EMBO J 22:1325–1335

    Article  PubMed  CAS  Google Scholar 

  • Luttik MAH, Overkamp KM, Kötter P, de Vries S, van Dijken P, Pronk JT (1998) The Saccharomyces cerevisiae NDE1 and NDE2 genes encode separate mitochondrial NADH dehydrogenases catalyzing the oxidation of cytosolic NADH. J Biol Chem 273:24529–24534

    Article  PubMed  CAS  Google Scholar 

  • Marres CAM, de Vries S, Grivell LA (1991) Isolation and inactivation of the nuclear gene encoding the rotenone-insensitive internal NADH:ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae. Eur J Biochem 195:857–862

    Article  PubMed  CAS  Google Scholar 

  • Mathiesen C, Hägerhall C (2003) The ‘antiporter module’ of respiratory chain Complex I includes the MrpC/NuoK subunit – a revision of the modular evolution scheme. FEBS Lett 549:7–13

    Article  PubMed  CAS  Google Scholar 

  • Matsuoka M, Matsubara M, Inoue J, Kakehi M, Imanaka T (1994) Organization and transcription of the mitochondrial ATP synthase genes in the yeast Yarrowia lipolytica. Curr Genet 26:382–389

    Article  PubMed  CAS  Google Scholar 

  • Medentsev AG, Akimenko VK (1999) Development and activation of cyanide-resistant respiration in the yeast Yarrowia lipolytica. Biochemistry 64:945–951

    PubMed  CAS  Google Scholar 

  • Melo AM, Bandeiras TM, Teixeira M (2004) New insights into type II NAD(P)H:quinone oxidoreductases. Microbiol Mol Biol Rev 68:603–616

    Article  PubMed  CAS  Google Scholar 

  • Montet Y, Amara P, Volbeda A, Vernede X, Hatchikian EC, Field MJ, Frey M, Fontecilla-Camps JC (1997) Gas access to the active site of Ni-Fe hydrogenases probed by X-ray crystallography and molecular dynamics. Nat Struct Biol 4:523–526

    Article  PubMed  CAS  Google Scholar 

  • Morgner N, Zickermann V, Kerscher S, Wittig I, Abdrakhmanova A, Barth HD, Brutschy B, Brandt U (2008) Subunit mass fingerprinting of mitochondrial complex I. Biochim Biophys Acta 1777:1384–1391

    Article  PubMed  CAS  Google Scholar 

  • Nosek J, Fukuhara H (1994) NADH dehydrogenase subunit genes in the mitochondrial DNA of yeasts. J Bacteriol 176:5622–5630

    PubMed  CAS  Google Scholar 

  • Nübel E, Wittig I, Kerscher S, Brandt U, Schägger H (2009) Two-dimensional native electrophoretic analysis of respiratory supercomplexes from Yarrowia lipolytica. Proteomics 9(9):2408–2418

    Article  PubMed  Google Scholar 

  • Ogata K, Volini M (1990) Mitochondrial rhodanese – membrane-bound and complexed activity. J Biol Chem 265:8087–8093

    PubMed  CAS  Google Scholar 

  • Ogilvie I, Kennaway NG, Shoubridge EA (2005) A molecular chaperone for mitochondrial complex I assembly is mutated in a progressive encephalopathy. J Clin Invest 115:2784–2792

    Article  PubMed  CAS  Google Scholar 

  • Ohnishi T (1998) Iron-sulfur clusters semiquinones in complex I. Biochim Biophys Acta 1364:186–206

    Article  PubMed  CAS  Google Scholar 

  • Okun JG, Lümmen P, Brandt U (1999) Three classes of inhibitors share a common binding domain in mitochondrial complex I (NADH:ubiquinone oxidoreductase). J Biol Chem 274:2625–2630

    Article  PubMed  CAS  Google Scholar 

  • Pagani S, Galante YM (1983) Interaction of rhodanese with mitochondrial NADH dehydrogenase. Biochim Biophys Acta 742:278–284

    Article  PubMed  CAS  Google Scholar 

  • Radermacher M, Ruiz T, Clason T, Benjamin S, Brandt U, Zickermann V (2006) The three-dimensional structure of complex I from Yarrowia lipolytica: a highly dynamic enzyme. J Struct Biol 154:269–279

    Article  PubMed  CAS  Google Scholar 

  • Rais I, Karas M, Schägger H (2004) Two-dimensional electrophoresis for the isolation of integral membrane proteins and mass spectrometric identification. Proteomics 4:2567–2571

    Article  PubMed  CAS  Google Scholar 

  • Roessler MM, King MS, Robinson AJ, Armstrong FA, Harmer J, Hirst J (2010) Direct assignment of EPR spectra to structurally defined iron-sulfur clusters in complex I by double electron–electron resonance. Proc Natl Acad Sci USA 107:1930–1935

    Article  PubMed  CAS  Google Scholar 

  • Sackmann U, Zensen R, Roehlen D, Jahnke U, Weiss H (1991) The acyl-carrier protein in Neurospora crassa mitochondria is a subunit of NADH: ubiquinone reductase (complex I). Eur J Biochem 200:463–469

    Article  PubMed  CAS  Google Scholar 

  • Sazanov LA, Hinchliffe P (2006) Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus. Science 311:1430–1436

    Article  PubMed  CAS  Google Scholar 

  • Sheftel AD, Stehling O, Pierik AJ, Netz DJA, Kerscher S, Elsasser HP, Wittig I, Balk J, Brandt U, Lill R (2009) Human Ind1, an iron-sulfur cluster assembly factor for respiratory complex I. Mol Cell Biol 29:6059–6073

    Article  PubMed  CAS  Google Scholar 

  • Smeitink JAM, van den Heuvel LWPJ, Koopman WJH, Nijtmans LGJ, Ugalde C, Willems PHGM (2004) Cell biological consequences of mitochondrial NADH: ubiquinone oxidoreductase deficiency. Curr Neurovasc Res 1:29–40

    Article  PubMed  CAS  Google Scholar 

  • Sokolova L, Wittig I, Barth HD, Schägger H, Brutschy B, Brandt U (2010) Laser-induced liquid bead ion desorption-MS of protein complexes from blue-native gels, a sensitive top-down proteomic approach. Proteomics 10:1401–1407

    Article  PubMed  CAS  Google Scholar 

  • Tocilescu MA, Fendel U, Zwicker K, Kerscher S, Brandt U (2007) Exploring the ubiquinone binding cavity of respiratory complex I. J Biol Chem 282:29514–29520

    Article  PubMed  CAS  Google Scholar 

  • Tocilescu MA, Fendel U, Zwicker K, Dröse S, Kerscher S, Brandt U (2010) The role of a conserved tyrosine in the 49-kDa subunit of complex I for ubiquinone binding and reduction. Biochim Biophys Acta 1797(6–7):625–632

    PubMed  CAS  Google Scholar 

  • Wada H, Shintani D, Ohlrogge J (1997) Why do mitochondria synthesize fatty acids? Evidence for involvement in lipoic acid production. Proc Natl Acad Sci USA 94:1591–1596

    Article  PubMed  CAS  Google Scholar 

  • Wittig I, Braun HP, Schägger H (2006) Blue native PAGE. Nat Protoc 1:418–428

    Article  PubMed  CAS  Google Scholar 

  • Yakovlev G, Reda T, Hirst J (2007) Reevaluating the relationship between EPR spectra and enzyme structure for the iron-sulfur clusters in NADH: quinone oxidoreductase. Proc Natl Acad Sci USA 104:12720–12725

    Article  PubMed  CAS  Google Scholar 

  • Yano T, Yagi T (1999) H+-translocating NADH-quinone oxidoreductase (NDH-1) of Paracoccus denitrificans. J Biol Chem 274:28606–28611

    Article  PubMed  CAS  Google Scholar 

  • Zickermann V, Bostina M, Hunte C, Ruiz T, Radermacher M, Brandt U (2003) Functional implications from an unexpected position of the 49 kDa subunit of complex I. J Biol Chem 278:29072–29078

    Article  PubMed  CAS  Google Scholar 

  • Zickermann V, Kerscher S, Zwicker K, Tocilescu MA, Radermacher M, Brandt U (2009) Architecture of complex I and its implications for electron transfer and proton pumping. Biochim Biophys Acta 1787:574–583

    Article  PubMed  CAS  Google Scholar 

  • Zickermann V, Angerer H, Ding MG, Nübel E, Brandt U (2010) Small single transmembrane domain (STMD) proteins organize the hydrophobic subunits of large membrane protein complexes. FEBS Lett 584(12):2516–2525

    Article  PubMed  CAS  Google Scholar 

  • Zwicker K, Galkin A, Dröse S, Grgic L, Kerscher S, Brandt U (2006) The redox-Bohr group associated with iron-sulfur cluster N2 of complex I. J Biol Chem 281:23013–23017

    Article  PubMed  CAS  Google Scholar 

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  1. Ulrich Brandt

    Present address: Nijmegen Centre for Mitochondrial Disorders (NCMD), Radboud University Medical Centre Geert Groteplein-Zuid 10, 6525 GA, Nijmegen, The Netherlands

Authors and Affiliations

  1. Center for Membrane Proteomics, Cluster of Excellence Frankfurt “Macromolecular Complexes”, Molekulare Bioenergetik, Fachbereich Medizin, Goethe-Universität, 60590, Frankfurt am Main, Germany

    Stefan Kerscher

  2. ZBC, Cluster of Excellence Frankfurt “Macromolecular Complexes”, Molekulare Bioenergetik, Fachbereich Medizin, Goethe-Universität, Frankfurt am Main, Germany

    Ulrich Brandt

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Correspondence to Ulrich Brandt .

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  1. Inst. Mikrobiologie, TU Dresden, Mommsenstr. 13, Dresden, 01062, Sachsen, Germany

    Gerold Barth

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Kerscher, S., Brandt, U. (2013). Mitochondrial Genomics and Proteomics of Yarrowia lipolytica . In: Barth, G. (eds) Yarrowia lipolytica. Microbiology Monographs, vol 24. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-38320-5_2

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