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Polypeptide composition of the globin poly(A)-protein complex from rabbit reticulocytes

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Abstract

Polypeptides associated with the rabbit reticulocyte poly(A)-protein complex, isolated by oligo (dT)-cellulose chromatography, were analyzed by SDS-polyacrylamide gel electrophoresis. The complex derived from EDTA released total polysomal mRNP and 20S globin mRNP by RNase treatment contained four polypeptides of molecular weight 58000, 67000, 71000 and 79000. A 79000 molecular weight polypeptide, which was also a major component of the reticulocyte low molecular weight cytoplasmic fraction, was shown to form tenacious associations with poly(A) in vitro.

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References

  1. KwanS-W. and BrawermanG., Proc. Nat. Acad. Sci. U.S.A. 69, 3247 (1972).

    Google Scholar 

  2. JefferyW. R. and BrawermanG., Biochemistry 13, 4633 (1974).

    Google Scholar 

  3. BlobelG., Proc. Nat. Acad. Sci. U.S.A. 70, 924 (1973).

    Google Scholar 

  4. BryanR.N. and HayashiM., Nature New Biol. 244, 271 (1973).

    Google Scholar 

  5. LindbergU. and SundquistB., J. Mol. Biol. 86, 451 (1974).

    Google Scholar 

  6. KumarA. and PedersonT., J. Mol. Biol. 96, 353 (1975).

    Google Scholar 

  7. IrwinD., KumarA., and MaltR.A., Cell, 4, 157 (1975).

    Google Scholar 

  8. GanderE.S., MuellerR.U., GoldenbergS., and MorelC., Mol. Biol. Rep. 2, 343 (1975).

    Google Scholar 

  9. BarrieuxA., IngrahamH.A., DavidD.N., and RosenfeldM., Biochemistry 14, 1815 (1975).

    Google Scholar 

  10. KishV.M. and PedersonT., J. Mol. Biol. 95, 227 (1975).

    Google Scholar 

  11. SchwartzH. and DarnellJ.E., J. Mol. Biol. 104, 833 (1976).

    Google Scholar 

  12. KishV.M. and PedersonT., J. Biol. Chem. 251, 5888 (1976).

    Google Scholar 

  13. JefferyW.R., J. Biol. Chem. 252, 3525 (1977).

    Google Scholar 

  14. LingrelJ.B., LockardR. E., JonesR. F., BurrH.E., and HoldenJ.W., Ser. Haemat. 4, 3 (1971).

    Google Scholar 

  15. HousmanD., Jacobs-LorenaM., RajbhandaryU. L., and LodishH. F., Nature 227, 913 (1970).

    Google Scholar 

  16. LaemmliU. K., Nature 227, 680 (1970).

    Google Scholar 

  17. HuezG., BurnyA., MarbaixG., and LeBleuB., Biochim. Biophys. Acta 145, 629 (1967).

    Google Scholar 

  18. SalasH. and GreenH., Nature New Biol. 229, 165 (1971).

    Google Scholar 

  19. HellermanJ.G. and ShafritzD.A., Proc. Nat. Acad. Sci. U.S.A. 72, 1021 (1975).

    Google Scholar 

  20. JefferyW. R. and BrawermanG., Biochemistry 14, 3445 (1975).

    Google Scholar 

  21. KishV.M. and PedersonT., Proc. Nat. Acad. Sci. U.S.A. 74, 1426 (1977).

    Google Scholar 

Download references

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Author notes

  1. Cynthia Peters

    Present address: Department of Zoology, University of Texas, 78712, Austin, Texas, USA

Authors and Affiliations

  1. Department of Biophysical Sciences, University of Houston, 77004, Houston, Texas, USA

    William R. Jeffery & Cynthia Peters

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  1. William R. Jeffery
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  2. Cynthia Peters
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Jeffery, W.R., Peters, C. Polypeptide composition of the globin poly(A)-protein complex from rabbit reticulocytes. Mol Biol Rep 3, 379–386 (1977). https://doi.org/10.1007/BF00420397

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  • DOI: https://doi.org/10.1007/BF00420397

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