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Preparation in undenatured form of the main protein bound to heterogeneous nuclear RNA in liver and hepatoma cells

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Abstract

Particles carrying heterogeneous nuclear RNA (30 S-particles) were prepared from rat liver and Zajdela hepatoma ascites cell nuclei after ultrasonic disruption. The ribonucleoprotein structures were disintegrated in the presence of 100 mM spermidine. Using chromatography on Sepharose-polyadenylate a protein component has been obtained which possessed high affinity for heterogeneous nuclear RNA, polyuridylate and polyadenylate, and double-stranded DNA. This protein was the main species of the ribonucleoprotein studied; it showed bands with molcular weights of 37000 and 40000 respectively in SDS gel electrophoresis. The RNA-binding proteins isolated from liver and hepatoma had identical molecular weights and the same affinity for Sepharose-polyadenylate used in the isolation.

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Abbreviations

hnRNA:

heterogeneous nuclear RNA

hnRNP:

ribonucleoprotein which contains hnRNA

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Authors and Affiliations

  1. Max-Planck-Institut für Biochemie, 8033, Martinsried, Germany

    A. Schweiger & G. Kostka

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  1. A. Schweiger
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  2. G. Kostka
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Schweiger, A., Kostka, G. Preparation in undenatured form of the main protein bound to heterogeneous nuclear RNA in liver and hepatoma cells. Mol Biol Rep 3, 353–359 (1977). https://doi.org/10.1007/BF00420394

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  • DOI: https://doi.org/10.1007/BF00420394

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