Summary
We have completed the analysis of a DNA segment of the chloroplast genome of Euglena gracilis Klebs, Z-strain, which links the 3′ end of the extra 16S rRNA gene with the 5′ end of the 16S rRNA gene of the rrn operon A. This region is a mosaic of several structural elements and contains an intact rrn interoperon spacer of 1,080 bp, an extra 5S rRNA gene, an open reading frame for 406 codons (ORF 406) which is flanked by short inverted repeats and a short direct repeat originating from the rrn interoperon spacer. It seems that a once complete rrn operon underwent in the past an insertion/deletion event leaving intact the 16S and 5S rRNA but totally excising the 16S-23S intergenic spacer and the 23S rRNA gene. Instead a protein coding gene of yet unknown function was inserted along with other structural elements.
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References
Cohen SW, Chang ACY, Hsu L (1972) Proc Natl Acad Sci USA 69:2110–2114
El-Gewely M, Helling RB, Dibbits JGTh (1984) Mot Gen Genet 194:432–443
Flamant F, Heizmann P, Nigon V (1984) Curr Genet 8:9–13
Graf L, Kössel H, Stutz E (1980) Nature 286:908–910
Graf L, Roux E, Stutz E, Kössel H (1982) Nucleic Acids Res 10:6369–6381
Hallick RB (1984) In: Buetow DE (ed) The Biology of Euglena, vol IV. Academic Press, New York, in press
Jenni B, Stutz E (1979) FEBS Lett 102:95–99
Jenni B, Fasnacht M, Stutz E (1981) FEBS Lett 125:175–179
Karabin GD, Narita JO, Dodd JR, Hallick RB (1983) J Biol Chem 258:14790–14796
Koller B, Delius H (1982a) Mot Gen Genet 188:305–308
Koller B, Delius H (1982b) FEBS Lett 140:198–202
Koller B, Delius H, Helling RB (1984) Plant Mot Biol 3:127–136
Kyte J, Doolittle RF (1982) J Mot Biol 157:105–132
Messing J, Vieira J (1982) Gene 19:269–276
Messing J, Crea R, Seeberg Ph (1980) Nucleic Acids Res 9:309–321
Ravel-Chapuis P, Flamant F, Nicolas P, Heizmann P, Nigon V (1984) Nucleic Acids Res 121:1039–1048
Roux E, Graf L, Stutz E (1983) Nucleic Acids Res 11:1957–1968
Sanger F, Coulson AR, Barrel BG, Smith AJH, Roe BA (1980) J Mot Biol 143:161–178
Schlunegger B, Fasnacht M, Stutz E, Koller B, Delius H (1983) Biochim Biophys Acta 739:114–121
Starlinger P (1977) Annu Rev Genet 11:103–126
Starr RC (1964) Am J Bot 51:1013–1044
Tait RC, Rodriguez RL, West RW (1980) J Biol Chem 255:813–815
Wartell RM, Reznikoff WS (1980) Gene 9:307–310
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Roux, E., Stutz, E. The chloroplast genome of Euglena gracilis: the mosaic structure of a DNA segment linking the extra 16S rRNA gene with the rrn operon A. Curr Genet 9, 221–227 (1985). https://doi.org/10.1007/BF00420315
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DOI: https://doi.org/10.1007/BF00420315