Summary
Expression of the thymidylate synthase gene (TMPI) of Saccharomyces cerevisiae increases during the late G1 phase of the cell cycle. Using a series of gene fusions, which have placed the Escherichia coli lacZ gene under transcriptional and translational control of different portions of the TMPI gene, we have demonstrated the existence of three different regions which are important for expression. One of these regions, which was localized to within 270 base pairs of the translation start codon, is involved in the periodic expression of TMPI transcript. A second region, the deletion of which resulted in reduced levels of TMPI expression, is at least partially encoded by DNA sequences between 270 and 377 base pairs upstream of the translation start codon. A third region, located within the N-terminal 112 codons of the TMPI gene, apparently encodes information involved in a post-translational control mechanism.
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References
Barclay BJ, Little JG (1978) Mol Gen Genet 160:33–40
Barclay BJ, Kunz BA, Little JG, Haynes RH (1982) Can J Biochem 60:172–194
Barclay BJ, Little JG (1981) Mol Gen Genet 181:279–281
Birnboim HC, Doly J (1979) Nucleic Acids Res 7:1513–1523
Bisson L, Thorner J (1977) J Bacteriol 132:44–50
Bisson L, Thorner J (1982) Genetics 102:341–359
Brendel M, Langjahr UG (1974) Mol Gen Genet 131:351–358
Carlson M, Botstein D (1982) Cell 28:145–154
Casadaban MJ, Chou J, Cohen SN (1980) J Bacteriol 143:971–980
Casadaban MJ, Martinez-Arias A, Shapira SK, Chou J (1983) Methods Enzymol 100:293–308
Greenwood MT, Calmels EM, Storms RK (1986) J Bacteriol 168:1336–1342
Grenson M (1969) Eur J Biochem 11:249–260
Grivell AR, Jackson JF (1968) J Gen Microbiol 54:307–317
Guarente G (1983) Methods Enzymol 101:181–191
Hereford LM, Osley MA, Ludwig JR, McLaughlin CS (1981) Cell 24:367–375
Hryniuk WM, Bertino JR (1971) Annu NY Acad Sci 186:330–342
Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168
Kunz BA (1982) Environ Mutagen 4:695–725
Koyama H, Ayusawa D, Seno T (1982) Mutat Res 105:433–438
Little JG, Haynes RH (1979) Mol Gen Genet 168:141–151
Mandel M, Higa A (1970) J Mol Biol 53:159–162
Maniatis T, Jeffrey A, Kleid DG (1975) Proc Natl Acad Sci USA 72:1184–1188
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Martinez-Arias AE, Casadaban MJ (1983) Mol Cell Biol 3:580–586
McMaster GK, Carmichael GG (1977) Proc Natl Acad Sci USA 74:4835–4838
McNeil JB, Storms RK, Friesen JD (1980) Curr Genet 2:17–25
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Nasmyth K (1985a) Cell 42:213–223
Nasmyth K (1985b) Cell 42:225–235
O'Donovan GA, Neuhard J (1970) Bacteriol Rev 34:278–343
Osley MA, Gould J, King S, Kane M, Hereford L (1986) Cell 45:537–544
Osley MA, Hereford L (1982) Proc Natl Acad Sci USA 79:7689–7693
Rose M, Botstein D (1983) Methods Enzymol 101:167–180
Russel DW, Jensen R, Zoller MJ, Burke J, Errede B, Smith M, Herskowitz I (1986) Mol Cell Biol 6:4281–4294
Southern EM (1975) J Mol Biol 98:503–517
Storms RK, McNeil JB, Khanderkar PS, An G, Parker J, Friesen JD (1979) J Bacteriol 140:73–82
Storms RK, Ord RW, Greenwood MT, Mirdimadi B, Chu FK, Belfort M (1984) Mol Cell Biol 4:2858–2864
Struhl K (1981) J Mol Biol 152:569–575
Taylor GR, Barclay BJ, Storms RK, Friesen JD, Haynes RH (1982) Mol Cell Biol 2:437–442
Taylor GR, Lagosky PA, Storms RK, Haynes RH (1987) J Biol Chem 262:5298–5307
Thomas PS (1983) Methods Enzymol 100:255–266
Toper R, Fath WW, Brendel M (1981) Mol Gen Genet 182:60–64
White JHM, Green SR, Barker DG, Dumas LB, Johnston LH (1987) Exp Cell Res 171:223–231
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Ord, R.W., McIntosh, E.M., Lee, L. et al. Multiple elements regulate expression of the cell cycle-regulated thymidylate synthase gene of Saccharomyces cerevisiae . Curr Genet 14, 363–373 (1988). https://doi.org/10.1007/BF00419994
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DOI: https://doi.org/10.1007/BF00419994