Summary
Proliferating tumor cells obtained from ovarian, mammary, and endometrial tumors in tissue culture were tested for the influence of proteohormones and steroid hormones on cellular DNA synthesis and cell growth. The gonadotropic hormones stimulated DNA synthesis of ovarian tumor cells by single administration, or in combination with cortisol, up to the 11-fold of the comparable controls. The hormone sensitivity of the cell lines was variable, resulting in individual reaction patterns. There was no correlation to the histological diagnosis of the primary tumors with respect to the grade of differentiation. The results suggest that ovarian tumor cells in tissue culture can maintain sensitivity to organotropic hormones. Compared to the ovarian carcinoma lines, mammary or endometrial tumor cells did not respond to a similar extent. Progesterone decreased DNA synthesis of endometrial carcinoma cells.
Zusammenfassung
An proliferierenden Tumorzellen in der Gewebekultur aus Ovarial-, Mamma- und Endometriumcarcinomen wurde der Einfluß von Proteohormonen und von Steroidhormonen auf DNA-Synthese und Zellwachstum untersucht. Die Gonadotropine stimulierten die DNA-Synthese bei alleiniger Anwendung oder in Kombination mit Cortisol bis zum 11 fachen der vergleichbaren Kontrollwerte. Die Hormonsensitivität der einzelnen Zell-Linien war unterschiedlich, so daß individuelle Reaktionsmuster resultierten. Es bestand keine Korrelation zum histologischen Befund des Differenzierungsgrades der Primärtumoren. Die Ergebnisse zeigen, daß Ovarialtumorzellen in der Gewebekultur für organotrope Hormone sensitiv sein können. Verglichen mit den Ovarialtumorzellinien reagierten Mamma-oder Endometrium-Carcinomzellen nicht in gleicher Weise. Progesteron verminderte die DNA-Synthese von Endometriumtumorzellen.
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Simon, W.E., Hölzel, F. Hormone sensitivity of gynecological tumor cells in tissue culture. J Cancer Res Clin Oncol 94, 307–323 (1979). https://doi.org/10.1007/BF00419290
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DOI: https://doi.org/10.1007/BF00419290