Summary
Following mating of a and α isogenic haploids we observe that the frequency of plasmid bearing cells, during selective growth, increases three fold. By examining the mitotic stability, the frequency of plasmid bearing cells during the cell cycle and the copy number of ARS1 plasmids in isogenic haploid and diploid cells, we show that the apparent stability of circular ARS1 plasmids in a/α cells is largely due to a diminished copy number in these cells. This observation is fully comprehensible with the model for plasmid segregation as presented by Murray and Szostak (1983). In order to account for the differences in copy numbers, a and a/a isogenic strains were compared. Likewise a number of mating type nonspecific sterile mutants were compared with the parental Ste+ strain. It seems that a diminished copy number is established when the MATa1/MATα2 regulatory system (Klar et al. 1981) is switched on, since the effect is observed in Sir− strains only.
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References
Barford JP, Hall RJ (1976) Exp Cell Res 102:276–284
Celniker SE, Campbell IL (1982) Cell 31:201–213
Chan CSM, Tye BK (1980) Proc Natl Acad Sci (USA) 77:6329–6333
Clarke L, Carbon J (1980) Nature (London) 287:504–509
Clewell DB, Helinski DR (1969) Proc Natl Acad Sci (USA) 80:3406–3410
Dani GM, Zakian V (1983) Proc Natl Acad Sci (USA) 80:3406–3410
DiNardo S, Voelkel K, Sternglanz R (1984) Proc Natl Acad Sci (USA) 81:2616–2620
Errede B, Cardillo TS, Sherman F, Dubois E, Deschamps J, Wiame JM (1980) Cell 22:427–436
Fangman WL, Hice RH, Siedziewska EC (1983) Cell 32:831–838
Futcher AB, Cox BS (1984) J Bacteriol 157:283–290
Gulløv K, Friis J, Bonven B (1981) Exp Cell Res 136:295–304
Hartwell LH (1980) J Cell Biol 85:811–822
Hsiao CL, Carbon J (1979) Proc Natl Acad Sci (USA) 76:3829–3833
Hyman BC, Cramer JH, Rownd RH (1982) Proc Natl Acad Sci (USA) 79:1578–1582
Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168
Jayaram M, Li YY, Broach JR (1983) Cell 34:95–104
Kikuchi Y (1983) Cell 35:487–493
Kingsman AJ, Clarke L, Mortimer RK, Carbon J (1979) Gene 7:141–152
Klar AJS, Strathern JN, Broach JR, Hicks JB (1981) Nature (London) 289:239–244
Miozzari G, Niederberger P, Hotter R (1978) J Bacteriol 134:48–59
Murray AW, Szostak JW (1983) Cell 34:961–970
Nasmyth KA, Tatchell K, Hall BD, Astell C, Smith M (1981) Nature (London) 289:244–250
Nasmyth KA (1982) Molecular genetics of yeast mating type. Annu Rev Genet 16:439–500
Newlon CS, Burke W (1980) Replication of small chromosomal DNAs in Yeast. In: Alberts B, Fox CF (eds) Mechanistic studies of DNA replication and recombination. Academic Press, New York London, p 399
Petersen JGL, Holmberg S, Nilson-Tillgren T, Kielland-Brandt MC (1983) Carlsberg Res Common 48:149–159
Stinchcomb DT, Struhl K, Davis RW (1979) Nature (London) 282:39–43
Stinchcomb DT, Mann C, Selker E, Davis RW (1981) DNA sequences that allow the replication and segregation of yeast chromosomes. In: Ray DS (ed) The initiation of DNA replication. Academic Press, New York London, p 473
Tschumper G, Carbon J (1980) Gene 10:157–166
Winston F, Chumley F, Fink GR (1983) Eviction and transplacement of mutant genes in yeast. Methods Enzymol 101:211–228
Zakian VA, Scott JF (1982) Mol Cell Biol 2:221–232
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Gulløv, K., Friis, J. Maintenance and copy number control of ARS1 plasmids in Saccharomyces cerevisiae . Curr Genet 10, 21–27 (1985). https://doi.org/10.1007/BF00418489
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DOI: https://doi.org/10.1007/BF00418489