Summary
Inhibition of thymidylate biosynthesis has been found to induce deletion of a LEU2 insert from the ribosomal DNA gene cluster of haploid strains of Saccharomyces cerevisiae. Loss of the insert was detected phenotypically by the enhanced production of both sectored (leu+/leu−) and non-sectored (leu−) colonies. Hybridization patterns obtained by Southern blot analysis of DNA from the leu+ and leu− sectors were consistent with the occurrence of unequal sister chromatid recombination. The induction of sectored colonies was prevented by the rad52-1 mutation but not by defects in RAD6. However, the formation of non-sectored leu− colonies was induced by thymidylate depletion in both rad52-1 and rad6 strains.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barclay BJ, Kunz BA, Little JG, Haynes RH (1982) Can J Biochem 60:172–194
Bisson L, Thorner J (1977) J Bacteriol 132:44–50
Blakley RL (1969) The biochemistry of folic acid and related pteridines. North Holland, Amsterdam
Eckardt F, Kunz BA, Haynes RH (1981) Environ Mutagen 3:318–319
Eckardt F, Kunz BA, Haynes RH (1983) Curr Genet 7:399–402
Grivell AR, Jackson JF (1968) J Gen Microbiol 54:307–319
Haynes RH, Eckardt F, Kunz BA, Göggleman W (1982) The importance of mutant yield for the quantification of induced mutagenesis. In: Sugimura T, Kondo S, Takebe H (eds) Environmental mutagens and carcinogens. The University of Tokyo Press and Alan R. Liss, Inc, p 137
Haynes RH, Kunz BA (1981) DNA repair and mutagenesis in yeast. In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces, Life cycle and inheritance. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p 371
Jackson JA, Fink GR (1981) Nature 292:306–311
Kunz BA (1982) Environ Mutagen 4:695–725
Kunz BA, Barclay BJ, Game JC, Little JG, Haynes RH (1980) Proc Natl Acad Sci USA 77:6057–6061
Kunz BA, Eckardt F, Little JG, Haynes RH (1982) Curr Genet 5:29–31
Kunz BA, Haynes RH (1981) Ann Rev Genet 15:57–89
Kunz BA, Haynes RH (1982) Mutat Res 93:353–375
Little JG, Haynes RH (1979) Mol Gen Genet 168:141–151
Monteleone BA, Prakash S, Prakash L (1981) Mol Gen Genet 184:410–415
Petes TD (1980) Cell 19:765–774
Prakash L, Taillon-Miller P (1981) Curr Genet 3:247–250
Setlow RB, Setlow JK (1972) Ann Rev Biophys Bioeng 1:293–346
Southern EM (1975) J Mol Biol 98:503–517
Szostak JW, Wu R (1979) Plasmid 2:536–554
Szostak JW, Wu R (1980) Nature 284:426–430
Taylor GR, Barclay BJ, Storms RK, Friesen JD, Haynes RH (1982) Mol Cell Biol 2:437–442
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kunz, B.A., Taylor, G.R., Konforti, B. et al. Inhibition of thymidylate biosynthesis induces mitotic unequal sister chromatid recombination in Saccharomyces cerevisiae . Curr Genet 8, 211–217 (1984). https://doi.org/10.1007/BF00417818
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00417818