Summary
Histochemical findings of primary and metastatic amelanotic melanomas were shown by the formaldehyde-induced fluorescence method (Falck and Hillarp). All or some of the amelanotic melanoma cells were discovered to emit green specific fluorescence. Results of the determination of 5-S-cysteinyldopa and DOPA in amelanotic melanoma tissues indicated that the specific fluorescence emitted by these cells is primarily due to the presence of 5-S-cysteinyldopa. The values of 5-S-cysteinyldopa in these tissues were lower than those in melanotic melanoma, but were approximately the same as those in pigmented nevus. When unpigmented tumors were histopathologically revealed to be malignant, amelanotic melanoma could be definitely diagnosed by the fluorescence method of Falck and Hillarp and the biochemical analysis of 5-S-cysteinyldopa in the tissues.
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References
Agrup G, Björklund A, Falck B, Jacobsson S, Lindvall O, Rorsman H, Rosengren E (1977) Fluorescence histochemical demonstration of dopa thioethers by condensation with gaseous formaldehyde. Histochemistry 52:179–186
Agrup G, Agrup P, Andersson T, Hafström L, Hansson C, Jacobsson S, Jönsson P-E, Rorsman H, Rosengren A-M, Rosengren E (1979) Five years' experience of 5-S-cysteinyldopa in melanoma diagnosis. Acta Derm Venereol (Stockh) 59:381–388
Agrup G, Hansson C, Rorsman H, Rosengren A-M, Rosengren E (1980) Intracellular distribution of dopa and 5-S-cysteinyldopa in pigment cells with mininal pigment formation. Acta Derm Venereol (Stockh) 56:355–356
Anderson CW, Stevens MH, Moatamed F (1981) Electron microscopy and L-DOPA reaction in the evaluation of an unusual amelanotic malignant melanoma of the neck. Otolaryngol Head Neck Surg 89:594–598
Aubert Ch, Rosengren E, Rorsman H, Rouge F, Foa C, Lipcey C (1977) 5-S-Cysteinyldopa in diagnosis and treatment of human malignant melanomas and ultrastructural observations. Eur J Cancer 13:1299–1308
Björklund A, Falck B, Jacobsson S, Rorsman H, Rosengren A-M, Rosengren E (1972) Cysteinyldopa in human malignant melanoma. Acta Derm Venereol (Stockh) 52:357–360
Corrodi H, Jonsson G (1967) Fluorescence method for the histochemical demonstration of biogenic monoamines. J Histochem Cytochem 15:65–78
Falck B, Hillarp N-Å, Thieme G, Torp A (1962) Fluorescence of catecholamines and related compounds condensed with form-aldehyde. J Histochem Cytochem 10:348–354
Falck B, Jacobsson S, Olivercrona H, Rorsman H (1966) Fluorescent dopa reaction of nevi and melanoma. Arch Dermatol 94:363–369
Falck B, Jacobsson S, Olivercrona H, Olsen G, Rorsman H, Rosengren E (1966) Determination of catecholamines, 5-hydroxytryptamine and 3,4-dihydroxyphenylalanine (dopa) in human malignant melanomas. Acta Derm Vernereol (Stockh) 46:65–67
Falck B, Jacobsson S, Lindvall O, Nietsche U-B (1976) On the occurrence of cysteinyldopa and dopa in melanocytes and benign nevi cells. Scand J Plast Surg 10:185–188
Fitzpatrik TB (1952) Human melanogenesis. Arch Dermatol 65:379–391
Imagawa I, Endo M, Morishima T (1976) Mechanism of recurrence of pimented nevi following dermabrasion. Acta Derm Venereol (Stockh) 56:353–359
Morishima T (1970) Histochemical studies on the monoaminergic nerve in the skin chiefly by means of the fluorescence method of Falck and Hillarp. Acta Derm Venereol (Stockh) 50:329–337
Morishima T, Ishikawa T, Endo M, Tsujiguchi Y (1978) Pagetoid malignant melanoma and lentigo maligna melanoma of toe. A study with the fluorescence method (Falck and Hillarp). Arch Dermatol Res 262:275–283
Morishima T, Endo M (1978) Pigmented nevus between recipient and donor sites by intermediate skin grafting procedure. A study with fluorescence method (Falck and Hillarp). Acta Derm Venereol (stockh) 58:231–235
Morishima T, Tatsumi F, Fukada E, Saito M, Watanabe M, Nagashima N, Hanawa S (1983) Cysteinyldopa isomers and dopa in lesions and in urine of Japanese patients with malignant melanoma. Arch Dermatol Res 275:76–79
Paul E, Hartwig H-G, Möller W, Illig L (1975) Fluorescenz-histochemische Untersuchung und mikrofluorometrische Analysen an pigmentbildenden Tumoren der Haut. Arch Dermatol Forsch 253:125–144
Paul E, Illig L (1976) Melanin-producing dendritic cells and histogenesis of malignant melanoma. Arch Dermatol Res 257:163–177
Prota G (1980) Recent advances in the chemistry of melanogenesis in mammals. J Invest Dermatol 75:122–127
Rorsman H, Agrup G, Hansson C, Rosengren A-M, Rosengren E (1979) Detection of phaeomelanin. In: Klaus SN (ed) Pigment cell, vol 4. Karger, Basel, pp 244–252
Vogel CL, Rorsman H, Rosengren A-M, Rosengren E (1974) DOPA and 5-S-cysteinyldopa in malignant melanoma in Ugandan Africans. Acta Derm Venereol (Stockh) 54:19–20
Zelickson A-S (1962) The fine structure of the human melanotic and amelanotic malignant melanoma. J Invest Dermatol 39:605–613
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Morishima, T., Tatsumi, F., Fukada, E. et al. Studies on amelanotic melanoma with the fluorescence method (Falck and Hillarp) and biochemical analysis of 5-S-cysteinyldopa in the tissues. Arch Dermatol Res 275, 329–333 (1983). https://doi.org/10.1007/BF00417207
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DOI: https://doi.org/10.1007/BF00417207