Skip to main content
SpringerLink
Log in

Genetic and biochemical analyses of protein II

  • Section 2: Neisseria Genetics
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

We compared the structure of P.II proteins of gonococcal strain FA1090 by N-terminal sequence analysis of purified proteins and by DNA sequencing of cloned P.II genes. Regulation of P.II gene expression does not involve major DNA rearrangements, but may involve generation of frame-shifts in unexpressed P.II genes. There are probably 8 or 9 P.II genes, each possessing a common leader sequence, in the gonococcal chromosome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Black, W. J., R. S. Schwalbe, J. Nachamkin & J. G. Cannon (1984) Characterization of N. gonorrhoeae protein II phase variation by use of monoclonal antibodies. Infect. Immun. 45: 453–7

    Google Scholar 

  • Hagbloom, P., E. Segal, E. Billvard & M. So (1985) Intragenic recombination leads to pilus antigenic variation in N. gonorrhoeae. Nat. 315: 156–8

    Google Scholar 

  • Schwalbe, R., S. Cannon & J. G. Cannon (1986) Genetic transformation of genes for protein II in N. gonorrhoeae. J. Bact. 167: 186–90

    Google Scholar 

  • Schwalbe, R. S., W. J., Black, D. G., Klapper, J. G., Cannon (1985a) Genetics and chemistry of protein II. The pathogenic Neisseria: Proc. of the 4th Internatl. Symp. ASM, Wash., D.C.

  • Schwalbe, R. S., P. F. Sparling & J. G. Cannon (1985b) Variation of N. gonorrhoeae protein II among isolates from an outbreak caused by a single gonococcal strain. Infect. Immun. 49: 250–2

    Google Scholar 

  • Segal, E., E. Billyard, M. So, S. Störzbach, & T. F. Meyer (1985) Role of chromosomal rearrangement in N. gonorrhoeae pilus phase variation. Cell 40: 293–300

    Google Scholar 

  • Stern, A., P. Nickel, T. F. Meyer & M. So (1984) Opacity determinants of N. gonorrhoeae: gene expression and chromosomal linkage to the gonococcal pilus gene. Cell 37: 447–56

    Google Scholar 

  • Swanson, J. (1978) Studies on gonococcus infection. XIV. Cell protein differences among color/opacity colony variants of N. gonorrhoeae. Infect. Immun. 21: 292–302

    Google Scholar 

  • Swanson, J. & O. Barrera (1983) Immunological characteristics of gonococcal outer membrane protein II assessed by immunoprecipitation, immunoblotting, and coagglutination. J. Exp. Med. 157: 1405–20

    Google Scholar 

  • Swanson, J., S. Bergstrom, O. Barrera, K. Robbins & D. Corwin (1985) Pilus gonococcal variants. Evidence for multiple forms of piliation control. J. Exp. Med. 162: 729–44

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Microbiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    T. D. Connell, D. S. Barritt, T. H. Kawula, D. G. Klapper, A. Stephenson & J. G. Cannon

  2. Dept. of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA

    W. J. Black

  3. Laboratory Service, Albany VA Medical Center, Albany, NY, USA

    R. S. Schwalbe

Authors
  1. T. D. Connell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. S. Barritt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. J. Black
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. H. Kawula
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. G. Klapper
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R. S. Schwalbe
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. Stephenson
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. J. G. Cannon
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Connell, T.D., Barritt, D.S., Black, W.J. et al. Genetic and biochemical analyses of protein II. Antonie van Leeuwenhoek 53, 421–424 (1987). https://doi.org/10.1007/BF00415496

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00415496

Keywords

Search

Navigation