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Novel Type I Toxin-Antitoxins Loci

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Prokaryotic Toxin-Antitoxins

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Abstract

Although plasmid encoded type I TA loci have been known for many years, recent discoveries have identified novel, chromosomally encoded type I loci. Many novel type I families have now been identified but their exact biological roles are not known. Here, I describe their discovery, their distribution, and the possible functions for these unusual gene pairs.

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References

  • Argaman, L., Hershberg, R., Vogel, J., Bejerano, G., Wagner, E. G., Margalit, H., et al. (2001). Novel small RNA-encoding genes in the intergenic regions of Escherichia coli. Current Biology, 11, 941–950.

    Article  PubMed  CAS  Google Scholar 

  • Darfeuille, F., Unoson, C., Vogel, J., & Wagner, E. G. (2007). An antisense RNA inhibits translation by competing with standby ribosomes. Molecular Cell, 26, 381–392.

    Article  PubMed  CAS  Google Scholar 

  • Faridani, O. R., Nikravesh, A., Pandey, D. P., Gerdes, K., & Good, L. (2006). Competitive inhibition of natural antisense Sok-RNA interactions activates Hok-mediated cell killing in Escherichia coli. Nucleic Acids Research, 34, 5915–5922.

    Article  PubMed  CAS  Google Scholar 

  • Fozo, E. M., Hemm, M. R., & Storz, G. (2008a). Small toxic proteins and the antisense RNAs that repress them. Microbiology and Molecular Biology Reviews, 72, 579–589.

    Article  PubMed  CAS  Google Scholar 

  • Fozo, E. M., Kawano, M., Fontaine, F., Kaya, Y., Mendieta, K. S., Jones, K. L., et al. (2008b). Repression of small toxic protein synthesis by the sib and OhsC small RNAs. Molecular Microbiology, 70, 1076–1093.

    Article  PubMed  CAS  Google Scholar 

  • Fozo, E. M., Makarova, K. S., Shabalina, S. A., Yutin, N., Koonin, E. V., & Storz, G. (2010). Abundance of type I toxin-antitoxin systems in bacteria: searches for new candidates and discovery of novel families. Nucleic Acids Research, 38, 3743–3759.

    Article  PubMed  CAS  Google Scholar 

  • Gerdes, K., Thisted, T., & Martinussen, J. (1990). Mechanism of post-segregational killing by the hok/sok system of plasmid R1: Sok antisense RNA regulates formation of a hok mRNA species correlated with killing of plasmid-free cells. Molecular Microbiology, 4, 1807–1818.

    Article  PubMed  CAS  Google Scholar 

  • Gerdes, K., & Wagner, E. G. (2007). RNA antitoxins. Curr Opin Microbiol, 10, 117–124.

    Article  PubMed  CAS  Google Scholar 

  • Greenfield, T. J., Ehli, E., Kirshenmann, T., Franch, T., Gerdes, K., & Weaver, K. E. (2000). The antisense RNA of the par locus of pAD1 regulates the expression of a 33-amino-acid toxic peptide by an unusual mechanism. Molecular Microbiology, 37, 652–660.

    Article  PubMed  CAS  Google Scholar 

  • Greenfield, T. J., & Weaver, K. E. (2000). Antisense RNA regulation of the pAD1 par post-segregational killing system requires interaction at the 5′ and 3′ ends of the RNAs. Molecular Microbiology, 37, 661–670.

    Article  PubMed  CAS  Google Scholar 

  • Han, K., Kim, K. S., Bak, G., Park, H., & Lee, Y. (2010). Recognition and discrimination of target mRNAs by Sib RNAs, a cis-encoded sRNA family. Nucleic Acids Research, 38, 5851–5866.

    Article  PubMed  CAS  Google Scholar 

  • Hemm, M. R., Paul, B. J., Schneider, T. D., Storz, G., & Rudd, K. E. (2008). Small membrane proteins found by comparative genomics and ribosome binding site models. Molecular Microbiology, 70, 1487–1501.

    Article  PubMed  CAS  Google Scholar 

  • Hershberg, R., Altuvia, S., & Margalit, H. (2003). A survey of small RNA-encoding genes in Escherichia coli. Nucleic Acids Research, 31, 1813–1820.

    Article  PubMed  CAS  Google Scholar 

  • Hobbs, E. C., Astarita, J. L., & Storz, G. (2010). Small RNAs and small proteins involved in resistance to cell envelope stress and acid shock in Escherichia coli: Analysis of a bar-coded mutant collection. Journal of Bacteriology, 192, 59–67.

    Article  PubMed  CAS  Google Scholar 

  • Irnov, I., Sharma, C. M., Vogel, J., & Winkler, W. C. (2010). Identification of regulatory RNAs in Bacillus subtilis. Nucleic Acids Research, 38, 6637–6651.

    Article  PubMed  CAS  Google Scholar 

  • Jahn, N., Preis, H., Wiedemann, C., & Brantl, S. (2012). BsrG/SR4 from Bacillus subtilis–the first temperature-dependent type I toxin-antitoxin system. Molecular Microbiology, 83, 579–598.

    Article  PubMed  CAS  Google Scholar 

  • Kawano, M., Oshima, T., Kasai, H., & Mori, H. (2002). Molecular characterization of long direct repeat (LDR) sequences expressing a stable mRNA encoding for a 35-amino-acid cell-killing peptide and a cis-encoded small antisense RNA in Escherichia coli. Molecular Microbiology, 45, 333–349.

    Article  PubMed  CAS  Google Scholar 

  • Kawano, M., Reynolds, A. A., Miranda-Rios, J., & Storz, G. (2005). Detection of 5′- and 3′-UTR-derived small RNAs and cis-encoded antisense RNAs in Escherichia coli. Nucleic Acids Research, 33, 1040–1050.

    Article  PubMed  CAS  Google Scholar 

  • Mok, W. W., Patel, N. H., & Li, Y. (2010). Decoding toxicity: Deducing the sequence requirements of IbsC, a type I toxin in Escherichia coli. Journal of Biological Chemistry, 285, 41627–41636.

    Article  PubMed  CAS  Google Scholar 

  • Pedersen, K., & Gerdes, K. (1999). Multiple hok genes on the chromosome of Escherichia coli. Molecular Microbiology, 32, 1090–1102.

    Article  PubMed  CAS  Google Scholar 

  • Ramamurthi, K. S., Clapham, K. R., & Losick, R. (2006). Peptide anchoring spore coat assembly to the outer forespore membrane in Bacillus subtilis. Molecular Microbiology, 62, 1547–1557.

    Article  PubMed  CAS  Google Scholar 

  • Ramamurthi, K. S., Lecuyer, S., Stone, H. A., & Losick, R. (2009). Geometric cue for protein localization in a bacterium. Science, 323, 1354–1357.

    Article  PubMed  CAS  Google Scholar 

  • Rivas, E. R., Klein, J., Jones, T. A., & Eddy, S. R. (2001). Computational identification of noncoding RNAs in E. coli by comparative genomics. Current Biology, 11, 1369–1373.

    Article  PubMed  CAS  Google Scholar 

  • Rudd, K. E. (1999). Novel intergenic repeats of Escherichia coli K-12. Research in Microbiology, 150, 653–664.

    Article  PubMed  CAS  Google Scholar 

  • Saito, S., Kakeshita, H., & Nakamura, K. (2009). Novel small RNA-encoding genes in the intergenic regions of Bacillus subtilis. Gene, 428, 2–8.

    Article  PubMed  CAS  Google Scholar 

  • Sharma, C. M., Hoffmann, S., Darfeuille, F., Reignier, J., Findeiss, S., Sittka, A., et al. (2010). The primary transcriptome of the major human pathogen Helicobacter pylori. Nature, 464, 250–255.

    Article  PubMed  CAS  Google Scholar 

  • Silvaggi, J. M., Perkins, J. B., & Losick, R. (2005). Small untranslated RNA antitoxin in Bacillus subtilis. Journal of Bacteriology, 187, 6641–6650.

    Article  PubMed  CAS  Google Scholar 

  • Steglich, C., Futschik, M. E., Lindell, D., Voss, B., Chisholm, S. W., & Hess, W. R. (2008). The challenge of regulation in a minimal photoautotroph: Non-coding RNAs in Prochlorococcus. PLoS Genetics, 4, e1000173.

    Article  PubMed  Google Scholar 

  • Thisted, T., & Gerdes, K. (1992). Mechanism of post-segregational killing by the hok/sok system of plasmid R1. Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene. Journal of Molecular Biology, 223, 41–54.

    Article  PubMed  CAS  Google Scholar 

  • Unoson, C., & Wagner, E. G. (2007). Dealing with stable structures at ribosome binding sites: Bacterial translation and ribosome standby. RNA Biology, 4, 113–117.

    Article  PubMed  CAS  Google Scholar 

  • Unoson, C., & Wagner, E. G. (2008). A small SOS-induced toxin is targeted against the inner membrane in Escherichia coli. Molecular Microbiology, 70, 258–270.

    Article  PubMed  CAS  Google Scholar 

  • Vogel, J., Argaman, L., Wagner, E. G., & Altuvia, S. (2004). The small RNA IstR inhibits synthesis of an SOS-induced toxic peptide. Current Biology, 14, 2271–2276.

    Article  PubMed  CAS  Google Scholar 

  • Wassarman, K. M., Repoila, F., Rosenow, C., Storz, G., & Gottesman, S. (2001). Identification of novel small RNAs using comparative genomics and microarrays. Genes and Development, 15, 1637–1651.

    Article  PubMed  CAS  Google Scholar 

  • Waters, L. S., & Storz, G. (2009). Regulatory RNAs in bacteria. Cell, 136, 615–628.

    Article  PubMed  CAS  Google Scholar 

  • Weaver, K. E., Jensen, K. D., Colwell, A., & Sriram, S. I. (1996). Functional analysis of the Enterococcus faecalis plasmid pAD1-encoded stability determinant par. Molecular Microbiology, 20, 53–63.

    Article  PubMed  CAS  Google Scholar 

  • Weaver, K. E., Reddy, S. G., Brinkman, C. L., Patel, S., Bayles, K. W., & Endres, J. L. (2009). Identification and characterization of a family of toxin-antitoxin systems related to the Enterococcus faecalis plasmid pAD1 par addiction module. Microbiology, 155, 2930–2940.

    Article  PubMed  CAS  Google Scholar 

  • Weel-Sneve, R., Bjoras, M., & Kristiansen, K. I. (2008). Overexpression of the LexA-regulated tisAB RNA in E. coli inhibits SOS functions; implications for regulation of the SOS response. Nucleic Acids Research, 36, 6249–6259.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, University of Tennessee, Knoxville, TN, 37996, USA

    Elizabeth Fozo

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  1. Elizabeth Fozo
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Correspondence to Elizabeth Fozo .

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Editors and Affiliations

  1. Inst. for Cell and Molecular Bioscience, Centre for Bacterial Cell Biology, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, United Kingdom

    Kenn Gerdes

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Fozo, E. (2013). Novel Type I Toxin-Antitoxins Loci. In: Gerdes, K. (eds) Prokaryotic Toxin-Antitoxins. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-33253-1_3

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