Abstract
Intact spheroplasts of the cyanobacterium Anacystis nidulans were found to oxidize reduced c-type cytochromes derived from horse heart, tuna, Saccharomyces oviformis, Candida krusei, Rhodocyclus purpureus, Rhodopseudomonas plustris and Paracoccus denitrificans with characteristics similar to those observed with isolated membranes. Rates of cytochrome c oxidation by the spheroplasts were only 10% of those measured with isolated membranes in which thylakoid-bound cytochrome oxidase contributes to the overall rates. Small amounts of an endogenous c-type cytochrome were released upon lysozyme treatment of the cells. The results appear to indicate the presence of cytochrome oxidase in the cytoplasmic membrane of A. nidulans.
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Abbreviations
- CCCP:
-
carbonyl cyanide m-chlorophenylhydrazone
- DCCD:
-
N,N′-dicyclohexyl carbodiimide
- cyt:
-
cytochrome(s)
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Peschek, G.A., Schmetterer, G. & Wagesreiter, H. Oxidation of exogenous c-type cytochromes by intact spheroplasts of Anacystis nidulans . Arch. Microbiol. 133, 222–224 (1982). https://doi.org/10.1007/BF00415005
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DOI: https://doi.org/10.1007/BF00415005