Abstract
Growth and l-lactic acid production on 24 different carbohydrates and polyols (glycerol, mannitol and sorbitol) by Rhizopus arrhizus CCM 8109 were determined. The d- but not the l-forms of xylose, fructose, galactose, mannose, glucose, cellobiose, maltose and sucrose and partially hydrolysed starch were converted to l-lactic acid. Changes in lipid formation and fatty acid composition were detected in biomass grown on the different sugars. In the presence of polyols, growth and considerable production of lipids were observed with little or no lactate production. Invertase was mainly associated with the mycelium during growth on sucrose, whereas glucoamylase and α-amylase were produced extracellularly during growth on starch.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bligh, E.G. & Dyer, W.J. 1959 A rapid method of total lipids extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911–917.
Bok, S.H. & Demain, A.L. 1977 An improved colorimetric assay for polyols. Analytical Biochemistry 81, 18–20.
Christoferson, S.W. & Glass, R.L. 1969 Preparation of milk fat methyl esters by alcoholysis in a essentially monoalcoholic solution. Journal of Dairy Science 52, 1289–1290.
Hang, Y.D. & Yu, R. 1989 Kinetics of direct fermentation of agriculture commodities to l(+) lactic acid by Rhizopus oryzae. Biotechnology Letters 11, 597–600.
Kobayashi, K., Suginaka, H. & Yano, J. 1987 Analysis of fatty acid composition of Candida sp. by gas-liquid chromatography using a polar column. Microbios 51, 37–42.
Krištofíková, Ľ., Rosenberg, M., Vlnová, A., Šajbidor, J. & Čertík, M. 1991 Selection of Rhizopus strains for l(+)-lactic acid and γ-linolenic acid production. Folia Microbiologica 36, 451–455.
Losel, D.M. 1989 Function of lipids: specialized roles in fungi and algae. In Microbial Lipids, Vol. 2, eds Ratledge, C. & Wilkinson, S.G. pp. 367–438, London: Academic Press.
Polonský, J., Repášová, Ľ., Pekarovicová, I. & Košík, M. 1985 Isotachophoretic analysis of saccharidic acids. Journal of Chromatography 320, 111–118.
Prescott, S.C. & Dunn, C.G. 1959 Industrial Microbiology, 3rd edn. New York: McGraw-Hill.
Rosenberg, M., Švitel, J., Rosenbergová, I. & Šturdík, E. 1992a Importance of invertase activity for gluconic acid production from sucrose by Aspergillus niger. Acta Biotechnologica 12, 311–317.
Rosenberg, M., Krištofíková, Ľ., Proksa, B. & Magdolen, P. 1992b The formation of polyols and fatty acids during l-lactic acid fermentation by Rhizopus arrhizus. Biotechnology Letters 14, 45–48.
Sandhu, D.K., Vilkhu, K.S. & Soni, S.K. 1987 Production of α-amylase by Saccharomycopsis fibuligera (syn. Endomycopsis fibuligera). Journal of Fermentation Technology 65, 387–394.
Wilson, J.J. & Ingledev, W.M. 1982 Isolation and characterisation of Schwaniomyces alluvius amylolytic enzymes. Applied and Environmental Microbiology 44, 301–307.
Yu, R. & Hang, Y.D. 1990 Amylolytic enzyme production by Rhizopus oryzae grown on agricultural commodities. World Journal of Microbiology and Biotechnology 6, 15–18.
Additional information
The authors are with the Department of Biochemical Technology, Faculty of Chemical Technology, Slovak Polytechnical University, Radlinského 9, SK-812 37 Bratislava, Slovak Republic
Rights and permissions
About this article
Cite this article
Rosenberg, M., Krištofíková, Ľ. & Šturdík, E. Influence of carbohydrates and polyols on l-lactic acid production and fatty acid formation by Rhizopus arrhizus . World Journal of Microbiology & Biotechnology 10, 271–274 (1994). https://doi.org/10.1007/BF00414861
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00414861