Abstract
A thermophilic strain of Streptomyces thermonitrificans produced a high activity of intracellular glucose isomerase (12 U/ml) when grown in a medium containing 1% (w/v) xylose, supplemented with 2% (w/v) sorbitol as the second carbon source, at 50°C for 16 h. Addition of Mg2+ enhanced enzyme production but the organism could grow and produce the enzyme in the absence of Co2+.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bucke, C. 1977 Industrial glucose isomerase. In Topics in Enzyme and Fermentation Biotechnology, ed Wisemann, A. pp. 147–171. Chichester: Ellis Horwood.
Cllens, M., Kersters-Hilderson, H. & DeBruyne, C.K. 1985 d-Xylose isomerase from Streptomyces violaceus-ruber. Optimization of fermentation conditions. Biotechnology Letters 7, 597–600.
Chen, W.P., Anderson, A.W. & Han, Y.W. 1979 Production of glucose isomerase by Streptomyces flavogriseus. Applied and Environmental Microbiology 37, 324–331.
Chen, W.P. 1980 Glucose isomerase (a review). Process Biochemistry 15, 1–11.
Dische, Z. & Borenfreund, E. 1951 A new spectrophotometric method for the detection and determination of keto sugars and trioses. Journal of Biological Chemistry 192, 583–587.
Hafner, E.W. 1985 Constitutive producer of a thermostable glucose isomerase. US Patent 4551430. (Chemical Abstracts 104, 33063s.)
Kitada, M., Dobashi, Y. & Horikoshi, K. 1989 Enzymatic properties of purified d-xylose isomerase from a thermophilic alkalophile, Bacillus TX-3. Agricultural and Biological Chemistry 53, 1461–1468.
Marshall, R.O. & Kooi, E.R. 1957 Enzymatic conversion of d-glucose to d-fructose. Science 125, 648–649.
Rehm, H.J. & Reed, G. 1987 Production of enzymes by fermentation. In Biotechnology, Vol. 7a, ed Kennedy, J.F. pp. 172–211. Weinheim: Verlag-Chemie.
Takasaki, Y. 1974 Fructose from glucose by thermophilic Streptomyces. Japanese Patent 7442,555. (Chemical Abstracts 81, 123360c.)
Additional information
The authors are with the Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, India
NCL Communication No. 5813
Rights and permissions
About this article
Cite this article
Deshmukh, S.S., Deshpande, M.V. & Shankar, V. Medium optimization for the production of glucose isomerase from thermophilic Streptomyces thermonitrificans . World Journal of Microbiology & Biotechnology 10, 264–267 (1994). https://doi.org/10.1007/BF00414859
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00414859