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Isolation and characterization of a temperate cyanophage for a tropical Anabaena strain

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Abstract

In this paper we describe the isolation and characterization of a temperate cyanophage N(S)1 of the genus cyanopodovirus which produces turbid plaques on the host Anabaena 77S15 isolated from tropical soil. Its properties have been compared to those of other well-characterized cyanophages. In addition, two strains of Anabaena 77S15 lysogenic for N(S)1 were isolated. N(S)1 seems to be integrated into the chromosome of the two lysogens, and a 2 kb plasmid present at a low copy number in the non-lysogenic strain is amplified significantly.

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References

  • Adams MH (1959) Bacteriophages. Interscience Publishers Inc, New York

    Google Scholar 

  • Adolph KW, Haselkorn R (1971) Isolation and characterization of a virus infecting the blue-green alga Nostoc muscorum. Virology 46:200–208

    Google Scholar 

  • Brown RM Jr (1972) Algal viruses. Advan Virus Res 17:243–277.

    Google Scholar 

  • Cannon RE, Shane MS (1972) The effect of antibiotic stress on protein synthesis in the establishement of lysogeny of Plectonema boryanum. Virology 49:130–133

    Google Scholar 

  • Cannon RE, Shane MS, Bush VN (1971) Lysogeny of a blue-green alga Plectonema boryanum. Virology 45:149–153

    Google Scholar 

  • Currier TC, Wolk CP (1979) Characteristics of Anabaena variabilis influencing plaque formation by cyanophage N-1. J Bacteriol 139:88–92

    Google Scholar 

  • Durrel LW (1964) Algae in tropical soils. Trans Amer Micros Soc 83:79–85

    Google Scholar 

  • Eisenstark A (1967) Bacteriophage techniques. In: Maramorosch K, Koprowski H (eds) Methods in virology, vol 1. Academic Press, New York, pp 449–524

    Google Scholar 

  • Elmerich C, Quiviger B, Rosenberg C, Franche C, Laurent P, Döbereiner J (1982) Characterization of a temperate bacteriophage for Azospirillum. Virology 122:29–37

    Google Scholar 

  • Franche C (1984) Isolation and characterization of a new cyanophage infecting a tropical Nostoc strain. In: Veeger C, Newton WE (eds) Advances in nitrogen fixation research. Martinus Nijhoff, Junk Publ, The Hague, p 50

    Google Scholar 

  • Franche C, Reynaud PA (1986) Characterization of several tropical strains of Anabaena and Nostoc: morphological and physiological properties, and plasmid content. Ann Microbiol 137A: 179–197

    Google Scholar 

  • Gromov BV (1983) Cyanophages. Ann Microbiol 134:43–59

    Google Scholar 

  • Hu NT, Thiel T, Giddings TH, Wolk CP (1981) New Anabaena and Nostoc cyanophages from sewage settling ponds. Virology 114:236–246

    Google Scholar 

  • Humphreys GO, Willshow GA, Anderson ES (1975) A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta 383:457–463

    Google Scholar 

  • Khudyakov I, Gromov BV (1973) The temperate cyanophage A-4(L) of the blue-green alga Anabaena variabilis. Microbiologica 42:904–907

    Google Scholar 

  • Koz'yakov SY (1977) Cyanophages of the series A(L) specific for the blue-green alga Anabaena variabilis. Eksperimental' naya al' gologiya. Trudi Petergof Biol Inst (Leningrad State Univ) 25:151–175

    Google Scholar 

  • Luftig R, Haselkorn R (1967) Morphology of a virus of blue-green algae and properties of its deoxyribonucleic acid. J Virol 1:344–361

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory

  • Padan E, Shilo M (1973) Cyanophages. Viruses attacking blue-green algae. Bacteriol Reviews 37:343–370

    Google Scholar 

  • Padhy RN, Singh PK (1978) Lysogeny in the blue-green alga Nostoc muscorum. Arch Microbiol 117:265–268

    Google Scholar 

  • Quiviger B, Franche C, Lutfalla G, Rice D, Haselkorn R, Elmerich C (1982) Cloning of a nitrogen fixation (nif) gene cluster of Azospirillum brasilense. Biochimie 64:495–502

    Google Scholar 

  • Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61

    Google Scholar 

  • Safferman RS (1973) Phycoviruses. In: Carr NG, Whitton BA (eds) The biology of blue-green algae. Blackwell Sci Publ, Oxford, pp 214–237

    Google Scholar 

  • Safferman RS, Cammon RE, Desjardins PR, Gromov BV, Haselkorn R, Sherman LA, Shilo M (1983) Classification and nomenclature of viruses of cyanobacteria. Intervirology 19: 61–66

    Google Scholar 

  • Safferman RS, Morris ME (1964) Growth characteristics of the blue-green algal virus LPP-1. J Bacteriol 88:771–775

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Google Scholar 

  • Tel-Or E, Sandovsky T, Kobiler D, Arad H, Weinberg R (1983) The unique properties of the symbiotic Anabaena azollae in the water fern Azolla. In: Papageorgiou GC, Packer L (eds), Photosynthetic prokaryotes. Elsevier Science Publishing Co, New York, pp 303–314

    Google Scholar 

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Franche, C. Isolation and characterization of a temperate cyanophage for a tropical Anabaena strain. Arch. Microbiol. 148, 172–177 (1987). https://doi.org/10.1007/BF00414808

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  • DOI: https://doi.org/10.1007/BF00414808

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