Abstract
Several strains of four species of luminous marine bacteria were maintained in a chemostat at a constant dilution rate and a variety of steady state densities by carbon (glycerol) limitation in order to study the relationship between culture density and bioluminescence activity. In general, luminescence per cell was constant at high culture density, and decreased dramatically at low culture density. For Vibrio fischeri, luminescence decreased to nondectable levels when the culture was maintained at low density; such dark cells were stimulated to synthesize luciferase and became luminous within minutes when purified autoinducer was added to the chemostat. Two strains, Photobacterium phosphoreum NZ11D and Photobacterium leiognathi S1, did not show the decrease in light intensity at low culture density that was characteristic of all other strains tested; they appeared to be constitutive for bioluminescence.
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Abbreviations
- BCM:
-
basal salts glycerol medium
- BM:
-
basal salts medium
- BSA:
-
bovine serum albumin
- D:
-
dilution rate
- DTT:
-
dilitiothreitol
- LU:
-
light unit=2×1010 quanta s-1
- OD:
-
optical density
- SWC:
-
sea water complete medium
- μ:
-
specific growth rate
References
Baumann P, Baumann L, Bang SS, Woolkalis MJ (1980) Reevaluation of the taxonomy of Vibrio, Beneckea, and Photobacterium: abolition of the genus Beneckea. Curr Microbiol 4:127–132
Baldwin TO, Nicoli MZ, Reeve CA (1979) Bacterial luciferase: Proteolysis in vitro and inactivation in vivo. In: Cohen GN, Holzer H (eds) Limited Proteolysis in Microorganisms, DHEW Publication (NIH) 79-1591. U.S. Govt. Print. Off., Washington, D.C. pp 217–223
Bognar A, Michaliszyn G, Meighen E (1978) Differential synthesis of the polypeptides of aldehyde dehydrogenase and NAD(P)H: flavin oxidoreductase in the bioluminescent bacterium Beneckea harveyi. Can J Biochem 56:605–610
Eberhard A (1972) Inhibition and activation of bacterial luciferase synthesis. J Bacteriol 109:1101–1105
Eberhard A, Eberhard C, Burlingame AL, Kenyon GL, Oppenheimer NJ, Nealson KH (1981) Purification, identification and synthesis of Photobacterium fischeri autoinducer. In: DeLuca M, McElroy W (eds) A symposium on bioluminescence and chemiluminescence; basic chemistry and analytical applications. Academic Press, New York, pp. 113–120
Eccleston M, Kelly DP (1978) Oxidation kinetics and chemostat growth kinetics of Thiobacillus ferrooxidans on tetrathionate and thiosulfate. J Bacteriol 134:718–727
Greenberg EP, Hastings JW, Ulitzur S (1979) Induction of luciferase synthesis in Beneckea harveyi by other marine bacteria. Arch Microbiol 120:87–91
Hastings JW, Weber G (1963) Total quantum flux of isotopic sources. J Optical Soc Amer 53:1410–1415
Katznelson R, Ulitzur S (1977) Control of luciferase synthesis in a newly isolated strain of Photobacterium leiognathi. Arch Microbiol 115:347–351
Meighen EA, Bogacki IG, Bognar A, Michalisyn GA (1976) Induction of fatty acid aldehyde dehydrogenase activity during the development of bioluminescence in Beneckea harveyi. Biochem Biophys Res Commun 69:423–430
Michalisyn GA, Meighen EA (1976) Induced polypeptide synthesis during the development of bacterial bioluminescence. J Biol Chem 251:2541–2549
Nealson KH (1977) Autoinduction of bacterial luciferase: Occurrence, mechanism, and significance. Arch Microbiol 112:73–79
Nealson KH (1978) Isolation, identification and manipulation of luminous bacteria. Methods Enzymol 57:153–165
Nealson KH (1979) Alternative strategies of symbiosis of marine luminous fishes harboring light emitting bacteria. Trends Biochem Sci 4:105–110
Nealson KH, Hastings JW (1979) Bacterial bioluminescence: Its control and ecological significance. Microbiol Rev 43:496–518
Nealson KH, Platt T, Hastings JW (1970) Cellular control of the synthesis and activity of the bacterial luminescent system. J Bacteriol 104:313–322
Néeman Z, Ulitzur S, Branton D, Hastings JW (1977) Membrane polypeptides co-induced with the bacterial bioluminescent system. J Biol Chem 252:5150–5154
Ulitzur S, Hastings JW (1979) Autoinduction in luminous bacteria: A confirmation of the hypothesis. Curr Microbiol 2:345–348
Watanabe H, Mimura N, Takimoto A, Makamura T (1975) Luminescence and respiratory activities of Photobacterium phosphoreum: Competition for cellular reducing power. J Biochem 77:1147–1155
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Rosson, R.A., Nealson, K.H. Autoinduction of bacterial bioluminescence in a carbon limited chemostat. Arch. Microbiol. 129, 299–304 (1981). https://doi.org/10.1007/BF00414701
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DOI: https://doi.org/10.1007/BF00414701