Abstract
Urea is accumulated against a concentration gradient in Chlamydomonas reinhardii. Only energy generated from photosynthesis is used for this accumulation, while degradation of urea utilizes other energy sources. Exogenous supplied urea is distributed between two pools, one large nonmetabolic and one metabolic pool. Ammonia inhibits the transport from the nonmetabolic to the metabolic pool.
The nonmetabolic pool is probably located in the chloroplast, and the accumulation of urea is due to an active transport into the chloroplast.
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Abbreviations
- N medium:
-
medium without nitrogen source
- TCA:
-
trichloroacetic acid
- TCAI:
-
trichloroacetic acid insoluble fraction
References
Dains FB, Wertheim E (1920) The action of ammonia and amines on the substituted ureas and urethanes. II. Allophanic esters. J Am Chem Soc 42:2303–2309
Heber U (1974) Metabolite exchange between chloroplasts and cytoplasm. Ann Rev Plant Physiol 25:393–421
Hodson RC (1973) Nutritional control of urea metabolism in Chlamydomonas reinhardi (strain y-l). Plant physiol 51 (Suppl), 55
Hodson RC, Williams SK, Davidson WR (1975) Metabolic control of urea catabolism in Chlamydomonas reinhardi and Chlorella pyrenoidosa. J Bacteriol 121:1022–1035
Kuhl A, Lorenzen H (1964) Handling and culturing of Chlorella. In: Prescott DM (ed) Methods in Cell Physiology, vol 1. Academic Press, London New York, pp 159–188
Lien T, Knutsen G (1975) Inexpensive continuously operating centrifuge for rapid collection of microalgae. Lab Pract 24:29
Lien T, Knutsen G (1979) Synchronous growth of Chlamydomonas reinhardtii (Chlorophyceae): A review of optimal condition. J Phycol 15:191–200
Pettersen R (1975) Control by ammonia of intercompartmental guanine transport in Chlorella. Z Pflanzenphysiol 76:213–223
Raven JA (1974) Energetics of active phosphate influx in Hydrodictyon africanum. J exp Bot 25:221–229
Roon RJ, Levenberg B (1972) Urea amidolyase. I. Properties of the enzyme from Candida utilis. J Biol Chem 247:4107–4113
Selmer BL, Hodson RC, Williams SK, Howell SH (1975) The induction of allophanate lyase during the vegetative cell cycle in light-synchronized cultures of Chlamydomonas reinhardi. Biochim Biophys Acta 399:71–78
Stepka W (1957) Identification of amino acids by paper chromatography. In: Colowick CP, Kaplan NO (eds) Methods in enzymology Vol III. Academic Press, New York, pp 504–528
Subramamian KN, Weiss RL, Davis RH (1973) Use of external, biosynthetic, and organellar arginin by Neurospora. J Bacteriol 115:284–290
Tarantowicz-Marek E, Bralczyk J, Jankowski, J, Kleczkowski K (1972) Mechanism of urea decomposition by urease-less Torulopsis utilis. Acta Biochim Polon 19:325–331
Whitney PA, Cooper TG (1972) Urea carboxylase and allophanate hydrolase. Two components of adenosine triphosphate: urea amidolyase in Saccharomyces cerevisiae. J Biol Chem 247:1349–1353
Williams SK, Hodson RC (1977) Transport of urea at low concentrations in Chlamydomonas reinhardi. J Bacteriol 130:266–273
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Dagestad, D., Lien, T. & Knutsen, G. Degradation and compartmentalization of urea in Chlamydomonas reinhardii . Arch. Microbiol. 129, 261–264 (1981). https://doi.org/10.1007/BF00414694
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DOI: https://doi.org/10.1007/BF00414694