Abstract
Four recently described species of new genera of sulfate-reducing bacteria, Desulfobulbus propionicus, Desulfobacter postgatei, Desulfococcus multivorans and Desulfosarcina variabilis were examined with respect to adenylylsulfate reductase. All of the species examined contained the enzyme in sufficient concentrations to account for dissimilatory sulfate reduction.
Adenylylsulfate reductase was enriched 17.1-fold from Desulfobulbus propionicus by ammonium sulfate fractionation, ion exchange chromatography and gel filtration. The molecular weight was 175,000 and the enzyme contained 1 mol of flavin, 8 mol of non heme iron and 8 mol of labile sulfide per mol enzyme. Either ferricyanide or cytochrome c could be used as electron acceptors; the pH optimum was 7.7 with ferricyanide and 8.8 with cytochrome c.
K m values for AMP and sulfite were 90 μM and 1.3 μM with ferricyanide and 91 μM and 71 μM with cytochrome c as electron acceptor. K m values for ferricyanide and cytochrome c were 89 μM and 21 μM, respectively. The properties of the enzyme were compared with those of purified adenylylsulfate reductases from other microorganisms.
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Abbreviations
- APS:
-
adenylylsulfate
References
Adachi K, Suzuki I (1977) A study on the reaction mechanism of adenosine 5′-phosphosulfate reductase from Thiobacillus thioparus, and iron-sulfur flavoprotein. Can J Biochem 55:91–98
Bowen TJ, Happold FC, Taylor BF (1966) Studies on adenosine-5′-phosphosulfate reductase from Thiobacillus denitrificans. Biochim Biophys Acta 118:566–576
Bramlett RN, Peck HD Jr (1975) some physical and kinetic properties of adenyl sulfate reductase from Desulfovibrio vulgaris. J Biol Chem 250:2979–2986
Campbell LL Jr, Postgate JR (1965) Classification of the sporeforming sulfate-reducing bacteria. Bacteriol Rev 29:359–363
Ishimoto M (1959) Sulfate reduction in cell-free extracts of Desulfovibrio. J Biochem (Tokyo) 46:105–110
King TE, Morris RO (1966) Determination of acid-labile sulfide and sulfhydrylgroups. In: Estabrook RW, Pullman ME (eds) Methods in enzymology, vol 20. Academic Press Inc, New York, pp 635–641
Kirchhoff J, Trüper HG (1974) Adenylylsulfate reductase in Chlorobium limicola. Arch Microbiol 100:115–120
LeGall J, Postgate JR (1973) The physiology of sulfate-reducing bacteria. Adv Microbiol Physiol 10:81–133
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Lyric RM, Suzuki I (1970) Enzymes involved in the metabolism of thiosulfate by Thiobacillus thioparus. Properties of adenosine-5′-phosphosulfate reductase. Can J Biochem 48:344–354
Peck HD Jr (1959) The ATP-dependent reduction of sulfate with hydrogen in extracts of Desulfovibrio desulfuricans. Proc Natl Acad Sci USA 45:701–708
Peck HD Jr (1960) Adenosine-5′-phosphosulfate reductase as an intermediate in the oxidation of thiosulphate by Thiobacillus thioparus. Proc Natl Acad Sci USA 46:1053–1057
Peck HD Jr (1962) Symposium on metabolism of inorganic compounds. V. Comparative metabolism of inorganic sulfur compounds in microorganisms. Bacteriol Rev 26:67–94
Peck HD Jr, Deacon TE, Davidson JT (1965) Studies on adenosine-5′-phosphosulfate reductase from Desulfovibrio desulfuricans and Thiobacillus thioparus. I. The assay and purification. Biochim Biophys Acta 96:429–446
Peck HD Jr (1966) Some evolutionary aspects of inorganic sulfur metabolism. Lecture series on theoretical and applied aspects of modern microbiology. Univ of Maryland
Peck HD Jr (1974) The evolutionary significance of inorganic sulfur metabolism. Cambridge University Press, Cambridge
Postgate JR (1965) Recent advances in the study of the sulfate-reducing bacteria. Bacteriol Rev 29:425–441
Postgate JR, Campbell LL (1966) Classification of Desulfovibrio species, the nonsporulating sulfate-reducing bacteria. Bacteriol Rev 30:732–738
Rao NA, Felton SP, Huennekens FM (1967) Quantitative determination of mitochondrial flavins. In: Estabrook RW, Pullman ME (eds) Methods in enzymology, vol 10. Academic Press Inc, New York, pp 494–499
Siegel LM (1975) Biochemistry of the sulfur cycle. Metabolic Pathways 7:217–286
Thiele HH (1966) Wachstumsphysiologische Untersuchungen an Thiorhodaceae; Wasserstoffdonatoren und Sulfatreduktion. Dissertation, Univ Göttingen
Thiele HH (1968) Sulfur metabolism in Thiorhodaceae. V. Enzymes of sulfur metabolism in Thiocapsa floridans and Chromatium species. Antonie van Leeuwenhoek. J Microbiol Serol 34:350–356
Trüper HG, Peck HD Jr (1970) Formation of adenylyl sulfate reductase in phototrophic bacteria. Arch Mikrobiol 73:125–142
Trüper HG, Rogers LA (1971) Purification and properties of adenylyl sulfate reductase from the photosynthetic bacterium Thiocapsa roseopersicina. J Bacteriol 108:1112–1121
Trüper HG, Fischer U (1982) Anaerobic oxidation of sulphur compounds as electron donors for bacterial photosynthesis. Phil Trans R Soc Lond B 298:529–542
Widdel F (1980) Anaerober Abbau von Fettsäuren und Benzoesäure durch neu isolierte Arten Sulfat-reduzierender Bakterien. Dissertation, Univ Göttingen
Widdel F, Pfennig N (1977) A new anaerobic, sporing, acetate-oxidizing, sulfate-reducing bacterium, Desulfotomaculum acetoxidans. Arch Microbiol 112:119–122
Widdel F, Pfennig N (1981) Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. I. Isolation of new sulfate-reducing bacteria enriched with acetate from saline environments. Description of Desulfobacter postgatei gen. nov., sp. nov. Arch Microbiol 131:395–400
Widdel F, Pfennig N (1982) Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. II. Incomplete oxidation of propionate by Desulfobulbus propionicus gen. nov., sp. nov. Arch Microbiol 131:360–365
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Stille, W., Trüper, H.G. Adenylylsulfate reductase in some new sulfate-reducing bacteria. Arch Microbiol 137, 145–150 (1984). https://doi.org/10.1007/BF00414456
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DOI: https://doi.org/10.1007/BF00414456