Summary
The role of lysosomal enzymes in ischemic retinal pigment epithelial cells (RPE) of albino rabbits was examined with the modified Gomori technique for acid phosphatase. Ischemia was produced by cutting the lateral posterior ciliary artery and short posterior ciliary arteries (PCA).
Five days after PCA-cut RPE in the ischemic region were disorganized by increased enzymatic digestion.
In RPE of the border between the ischemic and normal region a lot of fragmented outer segments were phagocytosed 24 h to 7 days after PCA-cut. At this time phagosomes appeared much more frequently than in the normal retina, showing several variations in their shape, localization, and histochemical reaction. However, after 10 and 14 days, RPE in the border had a tendency to reduce the phagocytic activity. A strong acid phosphatase activity was encountered in the phagosomes of macrophages, which was considered to be derived from RPE, and seemed to play the major role in scavenging destructed retinal elements.
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References
Ansell, P.L., Marshall, J.: The distribution of extra-cellular acid phosphatase in the retina of retinitis pigmentosa rats. Exp. Eye Res. 19, 273–279 (1974)
Ansell, P.L., Marshall, J.: Laser induced phagocytosis in the pigment epithelium of the Hunter dystrophic rat. Br. J. Ophthalmol. 60, 819–828 (1976)
Bairati, A., Ozalesi, N.: The ultrastructure of the pigment epithelium and of the photorecptor-pigment epithelium junction in the human retina. J. Ultrastruct. Res. 9, 484–496 (1963)
de Duve, C., Beaufay, H.: Tissue fraction studies. 10. Influence of ischemia on the state of some bound enzymes in rat liver Biochem. J. 73, 610–616 (1959)
Hayasaka, S.: Distribution of lysosomal enzymes in the bovine eye. Jpn. J. Ophthalmol. 18, 233–239 (1974)
Hollyfield, J.G., Besharse, J.C., Rayborn, M.E.: The effect of light on the quantity of phagosomes in the pigment epithelium. Exp. Eye. Res. 23, 623–635 (1976)
Ishikawa, T., Yamada, E.: The degradation of the photoreceptor outer segment within the pigment epithelial cell of rat retina. J. Electron MIcrosc. (Tokyo). 19, 85–91 (1970)
Kikuchi, T.: Effect of circulatory disturbance of choroid on acid phosphatase activity. Acta. Soc. Ophthalmol. Jpn. 79, 1487–1501 (1975)
Kikuchi, T.: Effect of circulatory disturbance of choroid on acid phosphatase activity, 2. The origin of macrophage in repairing stage. Acta. Soc. Ophthalmol. Jpn. 80, 518–530 (1976)
Kikuchi, T., Takei, Y., Mizuno, K.: Histochemical studies on the distribution of acid phosphatase in the normal and ischemic retina. Jpn. J. Ophthalmol. 21, 318–334 (1977)
Marshall, J., Ansell, P.L.: Membranous inclusions in the retinal pigment epithelium: phagosomes and myeloid bodies. J. Anat. 110, 91–104 (1971)
Novikoff, A.A.: Lysosomes and related particles. In: The Cell II. 432–488. J. Brachetes and A.E. Mirsky (ed.). New Yorl: Academic Press 1961
Spitznas, M., Hogan, M.J.: Outer segments of photoreceptors and retinal pigment epithelium. Interrelationship in the human eye. Arch. Ophthalmol. 84, 810–819 (1970)
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Kikuchi, T., Mizuno, K. Phagocytic activity in the ischemic retinal pigment epithelial cells. Albrecht von Graefes Arch. Klin. Ophthalmol. 207, 83–90 (1978). https://doi.org/10.1007/BF00414305
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DOI: https://doi.org/10.1007/BF00414305