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Biochemical and genetic characteristics of the C4-dicarboxylic acids transport system of Salmonella typhimurium

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Summary

  1. 1.

    Two mutants of Salmonella typhimurium LT2, strains SM64 and SM 166, unable to use succinate, fumarate, or malate as carbon sources for growth, were isolated by treatment with N-methyl-N′-nitro-N-nitrosoguanidine.

  2. 2.

    Mutant cells, induced aerobically in any of the C4-dicarboxylic acids, presented all the enzymes of the Krebs cycle, as well as the phosphoenolpyruvate carboxykinase (required for growth in those substrates), with the same activities as those found in the parental LT2 strain.

  3. 3.

    The mutants presented a deficient transport system for the C4-dicarboxylic acids. The kinetics of induction of the transport system, as well as some of its characteristics, were studied in the parental and mutant strains. Also the chromosomal site dct, responsible for the deficiency observed in the mutants, was located and found to be cotransducible with locus xyl.

  4. 4.

    From biochemical and genetic studies it is concluded that in S. typhimurium the transport of the C4-dicarboxylic acids succinic, fumaric, and malic is carried out by a single transport system, common for the three substrates.

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Abbreviations

NG:

N-methyl-N′-nitro-N-nitrosoguanidine

DW:

dry weight

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Author notes

  1. José L. Parada

    Present address: Laboratorio de Genética, Laboratorios Pfizer, Buenos Aires, Capital Federal, Argentina

  2. Guillermo Carrillo-Castañeda

    Present address: Colegio de Graduados, Rama de Genética, Escuela Nacional de Agricultura, Chapingo, Edo. de México, México

Authors and Affiliations

  1. Departamento de Genética, Centro de Investigación y de Estudios Avanzados del IPN, México, D. F.

    José L. Parada, Manuel V. Ortega & Guillermo Carrillo-Castañeda

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  1. José L. Parada
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  2. Manuel V. Ortega
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  3. Guillermo Carrillo-Castañeda
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Parada, J.L., Ortega, M.V. & Carrillo-Castañeda, G. Biochemical and genetic characteristics of the C4-dicarboxylic acids transport system of Salmonella typhimurium . Archiv. Mikrobiol. 94, 65–76 (1973). https://doi.org/10.1007/BF00414078

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  • DOI: https://doi.org/10.1007/BF00414078

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