Summary
The effect of theophylline, an inhibitor of phosphodiesterase, on tyrosine (Tyr) transport across cell membrane was studied using cultured B-16 mouse melanoma cells. 1.5 mM Theophylline in culture medium increased Tyr uptake velocity in linear fashion up to 36h, after which it reached a plateau at which the cells showed about a 60% increase in Tyr transport velocity. This increase was independent of extracellular Na, essentially unaffected by cell density, and partially inhibited by concomitantly added cycloheximide. These results suggested that biosynthesis of macromolecules, probably acting as System L transporter, was induced by theophylline treatment.
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References
Fogh J, Fogh H (1964) A method for direct demonstration of pleuropneumonia-like organisms in cultured cells. Proc Soc Exp Biol Med 117:899–901
Guidotti GG, Borghetti AF, Gazzola GC (1978) The regulation of amino acid transport in animal cells. Biochim Biophys Acta 515:329–366
Kreider JW, Wade DR, Rosenthal M, Densley T (1975) Maturation and differentiation of B 16 melanoma cells induced by theophylline treatment. J Natl Cancer Inst 54:1457–1467
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Foline phenol reagent. J Biol Chem 193:265–275
Mason HS (1948) The chemistry of melanin. III. Mechanism of the oxidation of dihydroxyphenylalanine by tyrosinase. J Biol Chem 172:83–99
Oxender DL, Lee M, Cecchini G (1977) Regulation of amino acid transport activity and growth rate of animal cells in culture. J Biol Chem 252:2680–2683
Pariza MW, Butcher FR, Becker JE, Potter VR (1977) 3′, 5′-Cyclic AMP: independent induction of amino acid transport by epinephrine in primary cultures of adult rat liver cells. Proc Natl Acad Sci USA 74:234–237
Pomerantz SH (1966) The tyrosine hydroxylase activity of mammalian tyrosinase. J Biol Chem 241:161–168
Robinson JH (1976) Density regulation of amino acid transport in cultured, androgen-responsive tumor cells. J Cell Physiol 89:101–110
Saga K, Shimojo T (1982) Studies on the transport of tyrosine, leucine, and methionine in cultured B-16 mouse melanoma cells. J Biochem 92:343–355
Touabi M, Jeanrenaud B (1969) α-Aminoisobutyric acid uptake in isolated mouse fat cells. Biochim Biophys Acta 173:128–140
Wade DR, Burkart ME (1978) The role of adenosine 3′, 5′-cyclic monophosphate in the density-dependent regulation of growth and tyrosinase activity of B-16 melanoma cells. J Cell Physiol 94:265–274
Wong G, Pawelek J (1975) Melanocyte-stimulating hormone promotes activation of pre-existing tyrosinase molecules in Cloudman S91 melanoma cells. Nature 255:644–646
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Supported in part by Grant 58770747 from the Ministry of Education, Japan
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Saga, K., Shimojo, T. Effect of theophylline on the transport of tyrosine in cultured B-16 mouse melanoma cells. Arch Dermatol Res 276, 165–169 (1984). https://doi.org/10.1007/BF00414014
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DOI: https://doi.org/10.1007/BF00414014